Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli

Producing recombinant proteins is a major accomplishment of biotechnology in the past century. Heterologous hosts, either eukaryotic or prokaryotic, are used for the production of these proteins. The utilization of microbial host systems continues to dominate as the most efficient and affordable met...

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Bibliographic Details
Published in:Archives of microbiology 2024-04, Vol.206 (4), p.152-152, Article 152
Main Authors: Eskandari, Azadeh, Nezhad, Nima Ghahremani, Leow, Thean Chor, Rahman, Mohd Basyaruddin Abdul, Oslan, Siti Nurbaya
Format: Article
Language:English
Subjects:
Barriers
Biochemistry
Biomedical and Life Sciences
Biotechnology
Biotechnology - methods
Cell Biology
E coli
Ecology
Escherichia coli - metabolism
Escherichia coli Proteins - metabolism
Food industry
Host systems
Life Sciences
Metal ions
Microbial Ecology
Microbiology
Microorganisms
Optimization
Protein engineering
Proteins
Recombinant Proteins - metabolism
Review
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Summary:Producing recombinant proteins is a major accomplishment of biotechnology in the past century. Heterologous hosts, either eukaryotic or prokaryotic, are used for the production of these proteins. The utilization of microbial host systems continues to dominate as the most efficient and affordable method for biotherapeutics and food industry productions. Hence, it is crucial to analyze the limitations and advantages of microbial hosts to enhance the efficient production of recombinant proteins on a large scale. E. coli is widely used as a host for the production of recombinant proteins. Researchers have identified certain obstacles with this host, and given the growing demand for recombinant protein production, there is an immediate requirement to enhance this host. The following review discusses the elements contributing to the manifestation of recombinant protein. Subsequently, it sheds light on innovative approaches aimed at improving the expression of recombinant protein. Lastly, it delves into the obstacles and optimization methods associated with translation, mentioning both cis-optimization and trans-optimization, producing soluble recombinant protein, and engineering the metal ion transportation. In this context, a comprehensive description of the distinct features will be provided, and this knowledge could potentially enhance the expression of recombinant proteins in E. coli .
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-024-03871-2