Integrative Taxonomy of Prosogonotrema bilabiatum Vigueras, 1940 (Digenea: Sclerodistomidae): A Parasite in Atlantic Spadefish Chaetodipterus faber (Broussonet, 1782) (Acanthuriformes: Ephippidae) from Brazil

• Published in: Acta parasitologica 2024-03, Vol.69 (1), p.898-909
• Main Authors: Machado, A. B., Simões, R. O., Maldonado, A., Santos, M. A. J., Luque, J. L.
• Format: Article
• Language: English
• Subjects: Acanthuriformes; Animal Systematics/Taxonomy/Biogeography; Animals; Bayesian analysis; Biomedical and Life Sciences; Biomedicine; Brazil - epidemiology; Carbon dioxide; Chaetodipterus faber; Critical point; Dehydration; DNA, Helminth - genetics; Ecology; Ethanol; Fish Diseases - parasitology; Fishes - parasitology; Genetic markers; Histology; Light microscopy; Medical Microbiology; Microbiology; Microscopy, Electron, Scanning - veterinary; Molecular biology; Morphology; Optical microscopy; Original Paper; Parasites; Parasitology; Phylogenetics; Phylogeny; Primers (coatings); Prosogonotrema bilabiatum; RNA, Ribosomal, 28S - genetics; Scanning electron microscopy; Sclerodistomidae; Sequences; Statistical inference; Taxonomy; Tegument; Trematoda - anatomy & histology; Trematoda - classification; Trematoda - genetics; Trematoda - isolation & purification; Trematoda - ultrastructure; Trematode Infections - epidemiology; Trematode Infections - parasitology; Trematode Infections - veterinary
• ISSN: 1230-2821; 1896-1851; 1896-1851
• DOI: 10.1007/s11686-024-00825-y

Objectives The present work aims to expand the knowledge of the digenean species Prosogonotrema bilabiatum (Sclerodistomidae), a parasite of Chaetodipterus faber (Acanthuriformes) from Brazil, with an integrative taxonomic approach, using light microscopy, scanning electron microscopy, histology, and molecular biology. Methods Forty-one digenean specimens were stained with hydrochloric carmine for morphological studies. Eleven parasites were dehydrated through a graded ethanol series, critical point dried with carbon dioxide, and coated with gold for scanning electron microscopy analysis. Four specimens were processed following histological routine and stained with hematoxylin and eosin and Gomori trichrome. DNA extracted was amplified using 28S partial primer D1–D3. Maximum likelihood and Bayesian inference were performed for phylogenetic analysis. Results Morphometric and morphological data of the specimens studied ranged in accordance as observed in previous descriptions of the species. Observations from scanning electron microscopy and histology corroborated with those observed in stained whole mounts. Molecular analysis showed that specimens of P. bilabiatum from Brazil clustered with another two sequences of this species from different hosts and localities, with a high node support value. Conclusions The integrative taxonomic approach allowed to record and describe new characteristics of P. bilabiatum related to the tegument, the structure and the arrangement of its tissues. The use of molecular markers confirmed that specimens identified as P. bilabiatum from different hosts and localities are all conspecific. Further studies, mainly molecular with less conserved genetic markers, should be carried out to better understand the phylogenetic relationships of Prosogonotrema with Hemiuroidea.