Sargassum spp. Ethanolic Extract Elicits Toxic Responses and Malformations in Zebrafish (Danio rerio) Embryos

• Published in: Environmental toxicology and chemistry 2024-05, Vol.43 (5), p.1075-1089
• Main Authors: González‐Penagos, Carlos E., Zamora‐Briseño, Jesús Alejandro, Améndola‐Pimenta, Mónica, Cruz‐Quintana, Yanis, Santana‐Piñeros, Ana M., Torres‐García, Jesús R., Cañizares‐Martínez, Mayra A., Pérez‐Vega, Juan A., Peñuela‐Mendoza, Ana C., Rodríguez‐Canul, Rossanna
• Format: Article
• Language: English
• Subjects: Abnormalities; Animals; Bioassays; Chondrocytes; Chondrogenesis; Danio rerio; Differentiation; Edema; Embryo, Nonmammalian - drug effects; Embryos; Endochondral bone; Environmental impact; Ethanol - toxicity; Exposure; Extracellular matrix; Gas chromatography; Gas Chromatography-Mass Spectrometry; Gene regulation; Genes; Hemopoiesis; Histology; Malformations; Mass spectrometry; Mass spectroscopy; Morphogenesis; Morphology; Nervous system; Palmitic acid; Pentanone; Pigmentation; Sargassum; Sargassum - chemistry; Scoliosis; Toxicology; Transcriptome; Transcriptomes; Transcriptomics; Visual perception; Water Pollutants, Chemical - toxicity; Yolk sac; Zebrafish; Zebrafish embryo
• ISSN: 0730-7268; 1552-8618
• DOI: 10.1002/etc.5840

The amount of Sargassum spp. arriving in the Caribbean Sea has increased steadily in the last few years, producing a profound environmental impact on the ecological dynamics of the coasts of the Yucatan Peninsula. We characterized the toxicological effects of an ethanolic extract of Sargassum spp. on zebrafish (Danio rerio) embryos (ZFEs) in a 96‐h static bioassay using T1 (0.01 mg/L), T2 (0.1 mg/L), T3 (1 mg/L), T4 (10 mg/L), T5 (25 mg/L), T6 (50 mg/L), T7 (75 mg/L), T8 (100 mg/L), T9 (200 mg/L), and T10 (400 mg/L). In this extract, we detected 74 compounds by gas chromatography‐mass spectrometry (GC‐MS), of which hexadecanoic acid methyl ester, and 2‐pentanone 4‐hydroxy‐4‐methyl, were the most abundant. In ZFEs, a median lethal concentration of 251 mg/L was estimated. Exposed embryos exhibited extensive morphological changes, including edema in the yolk sac, scoliosis, and loss of pigmentation, as well as malformations of the head, tail, and eyes. By integrating these abnormalities using the Integrated Biological Response (IBRv2) and General Morphological Score (GMS) indices, we were able to determine that ZFEs exposed to 200 mg/L (T9) exhibited the most pronounced biological response in comparison with the other groups. In the comparative transcriptomic analysis, 66 genes were upregulated, and 246 genes were downregulated in the group exposed to 200 mg/L compared with the control group. In the upregulated genes, we identified several gene ontology‐enriched terms, such as response to xenobiotic stimuli, cellular response to chemical stimulus, transcriptional regulation, pigment metabolic process, erythrocyte differentiation and embryonic hemopoiesis, extracellular matrix organization, and chondrocyte differentiation involved in endochondral bone morphogenesis, among others. In the down‐regulated genes, we found many genes associated with nervous system processes, sensory and visual perception, response to abiotic stimulus, and the nucleoside phosphate biosynthetic process. The probable connections among the morphological changes observed in the transcriptome are thoroughly discussed. Our findings suggest that Sargassum spp. exposure can induce a wide negative impact on zebrafish embryos. Environ Toxicol Chem 2024;43:1075–1089. © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.