Unraveling Intracellular Protein Corona Components of Nanoplastics via Photocatalytic Protein Proximity Labeling

• Published in: Analytical chemistry (Washington) 2024-03, Vol.96 (12), p.4978-4986
• Main Authors: Zhang, Zhenduo, Dong, Xuepeng, Wan, Wang, Guo, Hengke, Sun, Rui, Feng, Huan, Wang, Mengdie, Wang, Zhiming, Jin, Hao, Sun, Jialu, Xia, Qiuxuan, Zhao, Qi, Shen, Di, Gao, Zhenming, Liu, Yu
• Format: Article
• Language: English
• Subjects: Alpha rays; analytical chemistry; Animals; Bioaccumulation; biosafety; Boron Compounds; chemical bonding; Composition; E coli; environmental sustainability; Escherichia coli; fluorescence; Hepatocytes; Iodine; Labeling; liver; Mice; Microplastics; Mitochondria; Nanomaterials; Nanoparticles; nanoplastics; Nanotechnology; Photocatalysis; Photooxidation; photosensitivity; Photosensitization; photosensitizing agents; Plastic pollution; Polystyrene; Polystyrene resins; Polystyrenes; Protein composition; Protein Corona; Proteins; Proteome; Proteomes; Proteomics; Proximity; Reactive oxygen species; Substrates
• ISSN: 0003-2700; 1520-6882; 1520-6882
• DOI: 10.1021/acs.analchem.4c00050

Bioaccumulation of nanoplastic particles has drawn increasing attention regarding environmental sustainability and biosafety. How nanoplastic particles interact with the cellular milieu still remains elusive. Herein, we exemplify a general approach to profile the composition of a “protein corona” interacting with nanoparticles via the photocatalytic protein proximity labeling method. To enable photocatalytic proximity labeling of the proteome interacting with particles, iodine-substituted BODIPY (I-BODIPY) is selected as the photosensitizer and covalently conjugated onto amino-polystyrene nanoparticles as a model system. Next, selective proximity labeling of interacting proteins is demonstrated using I-BODIPY-labeled nanoplastic particles in both Escherichia coli lysate and live alpha mouse liver 12 cells. Mechanistic studies reveal that the covalent modifications of proteins by an aminoalkyne substrate are conducted via a reactive oxygen species photosensitization pathway. Further proteomic analysis uncovers that mitochondria-related proteins are intensively involved in the protein corona, indicating substantial interactions between nanoplastic particles and mitochondria. In addition, proteostasis network components are also identified, accompanied by consequent cellular proteome aggregation confirmed by fluorescence imaging. Together, this work exemplifies a general strategy to interrogate the composition of the protein corona of nanomaterials by endowing them with photooxidation properties to enable photocatalytic protein proximity labeling function.