Dorsoventrally asymmetric expression of miR319/TCP generates dorsal-specific venation patterning in petunia corolla tube

Abstract Vein-associated pigmentation (venation) is a type of floral coloration adopted by plants to attract pollinators. Several petunia (Petunia hybrida) lines generate dorsoventrally asymmetric venation patterning of the corolla tube, in which venation is only present in the dorsal tube. The mole...

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Bibliographic Details
Published in:Journal of experimental botany 2024-06, Vol.75 (11), p.3401-3411
Main Authors: Zhang, Bin, Qin, Xiaoting, Han, Yao, Li, Mingyang, Guo, Yulong
Format: Article
Language:English
Subjects:
Flowers - genetics
Flowers - growth & development
Flowers - metabolism
Gene Expression Regulation, Plant
MicroRNAs - genetics
MicroRNAs - metabolism
Petunia - genetics
Petunia - growth & development
Petunia - metabolism
Plant Proteins - genetics
Plant Proteins - metabolism
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Summary:Abstract Vein-associated pigmentation (venation) is a type of floral coloration adopted by plants to attract pollinators. Several petunia (Petunia hybrida) lines generate dorsoventrally asymmetric venation patterning of the corolla tube, in which venation is only present in the dorsal tube. The molecular mechanism underlying this trait is unknown. Here, we demonstrate that miR319 is preferentially expressed in the dorsal corolla tube, leading to dorsoventrally asymmetric expression of its target genes. Transgenic lines overexpressing phy-miR319a generated uniform venation patterning of the corolla tube. Knockout of TCP genes targeted by miR319 promoted venation patterning in the ventral and dorsal tube, while overexpression of the miR319 target gene, PhTCP6, completely inhibited corolla tube venation patterning. In addition, miR319-targeted TCPs negatively regulated venation patterning, probably by repressing the regulator of venation patterning, AN4. Together, our data demonstrate that asymmetric expression of miR319 promotes venation patterning in the petunia dorsal tube alone by repressing the expression of its target TCP genes, which negatively regulate corolla tube venation patterning. These findings provide novel insights into how the dorsoventrally asymmetric distribution of venation patterning is established in zygomorphic flowers. miR319-targeted TCP genes repress floral venation patterning by reducing AN4 gene expression. Regulation of TCP genes by miR319 is responsible for venation patterning formation in petunia dorsal corolla tube.
ISSN:0022-0957
1460-2431
1460-2431
DOI:10.1093/jxb/erae127