Physiological evaluation of yeast strains under anaerobic conditions using glucose, fructose, or sucrose as the carbon source

• Published in: Journal of bioscience and bioengineering 2024-06, Vol.137 (6), p.420-428
• Main Authors: Andrade Silva, Cinthia Aparecida de, Oka, Marta Ligia, da Silva, Pedro Garcia Pereira, Honma, Janaina Mayumi, Leite, Rodrigo Simões Ribeiro, Fonseca, Gustavo Graciano
• Format: Article
• Language: English
• Subjects: Anaerobiosis; Biomass; Brazil; Carbon - metabolism; Ethanol - metabolism; Fermentation; Fructose; Fructose - metabolism; Glucose; Glucose - metabolism; Glycerol - metabolism; Kinetics; Kluyver effect; Principal component analysis; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Sucrose; Sucrose - metabolism; Yeasts - classification; Yeasts - growth & development; Yeasts - metabolism
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2024.02.003

The aim of this study was to evaluate the physiology of 13 yeast strains by assessing their kinetic parameters under anaerobic conditions. They included Saccharomyces cerevisiae CAT-1 and 12 isolated yeasts from different regions in Brazil. The study aimed to enhance understanding of the metabolism of these strains for more effective applications. Measurements included quantification of sugars, ethanol, glycerol, and organic acids. Various kinetic parameters were analyzed, such as specific substrate utilization rate (qS), maximum specific growth rate (μmax), doubling time, biomass yield, product yield, maximum cell concentration, ethanol productivity (PEth), biomass productivity, and CO2 concentration. S. cerevisiae CAT-1 exhibited the highest values in glucose for μmax (0.35 h−1), qS (3.06 h−1), and PEth (0.69 gEth L−1 h−1). Candida parapsilosis Recol 37 did not fully consume the substrate. In fructose, S. cerevisiae CAT-1 stood out with higher values for μmax (0.25 h−1), qS (2.24 h−1), and PEth (0.60 gEth L−1 h−1). Meyerozyma guilliermondii Recol 09 and C. parapsilosis Recol 37 had prolonged fermentation times and residual substrate. In sucrose, only S. cerevisiae CAT-1, S. cerevisiae BB9, and Pichia kudriavzevii Recol 39 consumed all the substrate, displaying higher PEth (0.72, 0.51, and 0.44 gEth L−1 h−1, respectively) compared to other carbon sources. [Display omitted] •The physiology of 13 yeasts was investigated under anaerobic conditions using glucose, fructose, or sucrose.•Saccharomyces cerevisiae CAT-1 showed the higher fermentation capacity and rapid substrate consumption.•Candida glabrata Recol 43 also exhibited favourable metabolic traits and high ethanol productivity.•Only S. cerevisiae CAT-1, S. cerevisiae BB9, and Pichia kudriavzevii Recol 39 consumed all the sucrose.•All yeast strains exhibited higher ethanol yields when using fructose as a substrate.