The actinomycete Kitasatospora sp. SeTe27, subjected to adaptive laboratory evolution (ALE) in the presence of selenite, varies its cellular morphology, redox stability, and tolerance to the toxic oxyanion

• Published in: Chemosphere (Oxford) 2024-04, Vol.354, p.141712-141712, Article 141712
• Main Authors: Firrincieli, Andrea, Tornatore, Enrico, Piacenza, Elena, Cappelletti, Martina, Saiano, Filippo, Pavia, Francesco Carfì, Alduina, Rosa, Zannoni, Davide, Presentato, Alessandro
• Format: Article
• Language: English
• Subjects: Actinomycete; Adaptive laboratory evolution; Kitasatospora; Oxidative stress; Selenite; Whole-genome sequencing
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2024.141712

The effects of oxyanions selenite (SeO32−) in soils are of high concern in ecotoxicology and microbiology as they can react with mineral particles and microorganisms. This study investigated the evolution of the actinomycete Kitasatospora sp. SeTe27 in response to selenite. To this aim, we used the Adaptive Laboratory Evolution (ALE) technique, an experimental approach that mimics natural evolution and enhances microbial fitness for specific growth conditions. The original strain (wild type; WT) isolated from uncontaminated soil gave us a unique model system as it has never encountered the oxidative damage generated by the prooxidant nature of selenite. The WT strain exhibited a good basal level of selenite tolerance, although its growth and oxyanion removal capacity were limited compared to other environmental isolates. Based on these premises, the WT and the ALE strains, the latter isolated at the end of the laboratory evolution procedure, were compared. While both bacterial strains had similar fatty acid profiles, only WT cells exhibited hyphae aggregation and extensively produced membrane-like vesicles when grown in the presence of selenite (challenged conditions). Conversely, ALE selenite-grown cells showed morphological adaptation responses similar to the WT strain under unchallenged conditions, demonstrating the ALE strain improved resilience against selenite toxicity. Whole-genome sequencing revealed specific missense mutations in genes associated with anion transport and primary and secondary metabolisms in the ALE variant. These results were interpreted to show that some energy-demanding processes are attenuated in the ALE strain, prioritizing selenite bioprocessing to guarantee cell survival in the presence of selenite. The present study indicates some crucial points for adapting Kitasatospora sp. SeTe27 to selenite oxidative stress to best deal with selenium pollution. Moreover, the importance of exploring non-conventional bacterial genera, like Kitasatospora, for biotechnological applications is emphasized. [Display omitted] •The rare actinomycete Kitasatospora sp. SeTe27 strain is notably tolerant to selenite.•Adaptive Laboratory Evolution (ALE) enhances selenite microbial resistance.•The ALE variant shows increased selenite uptake and redox stability.•The ALE strain acquires missense mutations on antibiotic-related genes.•Attenuation of metabolic processes favors bacterial survival under selenite pressure.