Biodegradation of sulfanilic acid by Pseudomonas paucimobilis

An aerobic bacterium, isolated from a contaminated site, was able to degrade sulfanilic acid (4-aminobenzenesulfonic acid) and was identified as Pseudomonas paucimobilis. The isolate could grow on sulfanilic acid (SA) as its sole carbon and nitrogen source and metabolized the target compound to biom...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2001, Vol.55 (1), p.101-107
Main Authors: PEREI, K, RAKHELY, G, KISS, I, POLYAK, B, KOVACS, K. L
Format: Article
Language:English
Subjects:
4-Aminobenzenesulfonic acid
Acids
Biodegradation
Biodegradation, Environmental
Biological and medical sciences
Biological treatment of waters
Biotechnology
Biotechnology - methods
Cells, Immobilized
Contaminants
Culture Media
Environment and pollution
Freshwater
Fundamental and applied biological sciences. Psychology
Government subsidies
Industrial applications and implications. Economical aspects
Industrial pollution
Industrial wastes
Industrial wastewater
Pseudomonas - growth & development
Pseudomonas - metabolism
Pseudomonas paucimobilis
Substrate Specificity
sulfanilic acid
Sulfanilic Acids - metabolism
Waste Disposal, Fluid - methods
Water Microbiology
Water Pollution, Chemical
Water Purification - methods
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Summary:An aerobic bacterium, isolated from a contaminated site, was able to degrade sulfanilic acid (4-aminobenzenesulfonic acid) and was identified as Pseudomonas paucimobilis. The isolate could grow on sulfanilic acid (SA) as its sole carbon and nitrogen source and metabolized the target compound to biomass. The bioconversion capacity depended on the sulfanilic acid concentration; greater than 98% elimination of the hazardous compound was achieved at low (10 mM) sulfanilic acid concentration, and the yield was greater than 70% at 50 mM concentration of the contaminant. The maximum conversion rate was 1.5 mmol sulfanilic acid/h per mg wet cells at 30 degrees C. Ca-alginate-phytagel proved a good matrix for immobilization of P. paucimobilis, with essentially unaltered biodegradation activity. Removal of sulfanilic acid from contaminated industrial waste water was demonstrated. SDS-PAGE analysis of the crude extract revealed novel proteins appearing upon induction with sulfanilic acid and related compounds, which indicated alternative degradation mechanisms involving various inducible enzymes.
ISSN:0175-7598
1432-0614
DOI:10.1007/s002530000474