special reactor design for investigations of mixing time effects in a scaled-down industrial L-lysine fed-batch fermentation process

• Published in: Biotechnology and bioengineering 1999-09, Vol.64 (5), p.599-606
• Main Authors: Schilling, B.M, Pfefferle, W, Leuchtenberger, W, Deckwer, W.D
• Format: Article
• Language: English
• Subjects: amino acid; Batch cell culture; Biological and medical sciences; biological production; Biomass; Bioreactors; Biosynthesis; Biotechnology; citrate synthase; Corynebacterium glutamicum; Enzymes; Equipment testing; Fermentation; Fundamental and applied biological sciences. Psychology; Industrial applications; L-lysine; L-lysine biosynthesis; L-lysine production; lysine; Methods. Procedures. Technologies; Microorganisms; Mixing; reactor design; scaledown; Various methods and equipments
• ISSN: 0006-3592; 1097-0290
• DOI: 10.1002/(SICI)1097-0290(19990905)64:5<599::AID-BIT10>3.0.CO;2-C

A specially designed model reactor based on a 42-L laboratory fermentor was equipped with six stirrers (Rushton turbines) and five cylindrical disks. In this model reactor, the mixing time, theta90, turned out to be 13 times longer compared with the 42-L standard laboratory fermentor fitted with two Rushton turbines and four wall-fixed longitudinal baffles. To prove the suitability of the model reactor for scaledown studies of mixing-time-dependent processes, parallel exponential fed-batch cultivations were carried out with the leucine-auxotrophic strain, Corynebacterium glutamicum DSM 5715, serving as a microbial test system. L-Leucine, the process-limiting substrate, was fed onto the liquid surface of both reactors. Cultivations were conducted using the same inoculum material and equal oxygen supply. The model reactor showed reduced sugar consumption (-14%), reduced ammonium consumption (-19%), and reduced biomass formation (-7%) which resulted in a decrease in L-lysine formation (-12%). These findings were reflected in less specific enzyme activity, which was determined for citrate synthase (CS), phosphoenolpyruvate carboxylase (PEP-C), and aspartate kinase (AK). The reduced specific activity of CS correlated with lower CO2 evolution (-36%) during cultivation. The model reactor represents a valuable tool to simulate the conditions of poor mixing and inhomogeneous substrate distribution in bioreactors of industrial scale.