Covalent Attachment of Cell-Adhesive, (Arg-Gly-Asp)-Containing Peptides to Titanium Surfaces

A three-step reaction procedure was applied to introduce RGD-containing peptides on the titanium surface. Water−vapor−plasma-pretreated titanium surfaces were first silanized with (3-aminopropyl)triethoxysilane, resulting in a multilayer film of poly(3-aminopropyl)siloxane. In a second reaction step...

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Bibliographic Details
Published in:Langmuir 1998-09, Vol.14 (19), p.5507-5516
Main Authors: Xiao, Shou-Jun, Textor, Marcus, Spencer, Nicholas D, Sigrist, Hans
Format: Article
Language:English
Subjects:
Amino acids
Biological and medical sciences
Cell immobilization
Crosslinking
Fundamental and applied biological sciences. Psychology
Molecular biophysics
Multilayers
Plastic films
Silanes
Silicones
Surface properties. Adsorption
Surface treatment
Titanium
X ray photoelectron spectroscopy
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Summary:A three-step reaction procedure was applied to introduce RGD-containing peptides on the titanium surface. Water−vapor−plasma-pretreated titanium surfaces were first silanized with (3-aminopropyl)triethoxysilane, resulting in a multilayer film of poly(3-aminopropyl)siloxane. In a second reaction step, the free primary amino groups were linked to one of the three hetero-cross-linkers:  N-succinimidyl-6-maleimidylhexanoate, N-succinimidyl-3-maleimidylpropionate, and N-succinimidyl trans-4-(maleimidylmethyl)cyclohexane-1-carboxylate. Onto the resulting terminal-maleimide surface, two model, cell-adhesive peptides, H-Gly-Arg-Gly-Asp-Ser-Pro-Cys-OH and H-Arg-Gly-Asp-Cys-OH were immobilized through covalent addition of the cysteine thiol (−SH) group. X-ray photoelectron spectroscopy, infrared reflection absorption spectroscopy, and radiolabeling techniques were applied to characterize the surfaces. From independent quantitative analysis, an approximate coverage of 0.2∼0.4 peptides/nm2 was calculated.
ISSN:0743-7463
1520-5827
DOI:10.1021/la980257z