Development and design of a 'ready-to-use'reaction plate for a PCR-based simultaneous detection of animal species used in foods

Different TaqManTM-polymerase chain reaction systems have been developed, which allow the detection of even minute amounts of beef, pork, lamb, goat, chicken, turkey and duck in processed foods. The species-specific systems are able to amplify DNA regions with no more than 108bp in size (exception:...

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Bibliographic Details
Published in:International journal of food science & technology 2007-01, Vol.42 (1), p.9-17
Main Authors: Laube, Ines, Zagon, Jutta, Spiegelberg, Almuth, Butschke, Andreas, Kroh, Lothar W, Broll, Hermann
Format: Article
Language:English
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Summary:Different TaqManTM-polymerase chain reaction systems have been developed, which allow the detection of even minute amounts of beef, pork, lamb, goat, chicken, turkey and duck in processed foods. The species-specific systems are able to amplify DNA regions with no more than 108bp in size (exception: duck, 212bp) located on the single-copy genes cyclic guanosine monophosphate (cyclic GMP) phosphodiesterase, ryanodine receptor and interleukin-2 precursor. The parallel detection of the common ingredient 'meat' produced from mammals and poultry was based on the amplification of a region of the myostatin gene. The limit of detection was determined to be ten genome copies for each system. The relative SD under repeatability condition was below 30%. In addition, a 'ready-to-use'reaction plate has been developed, which makes it possible to investigate the presence of the seven animal species in parallel after a single real-time run.
ISSN:0950-5423
DOI:10.1111/j.1365-2621.2006.01154.x