Arabidopsis calcineurin B‐like‐interacting protein kinase 8 and its functional homolog in tomato negatively regulates ABA‐mediated stomatal movement and drought tolerance

• Published in: Plant, cell and environment cell and environment, 2024-07, Vol.47 (7), p.2396-2409
• Main Authors: Liu, Fei, Liu, Qi, Wu, Ju‐Hua, Wang, Zong‐Qi, Geng, Yuan‐Jun, Li, Juan, Zhang, Yan, Li, Sha
• Format: Article
• Language: English
• Subjects: abiotic stress; Abscisic acid; Abscisic Acid - metabolism; Abscisic Acid - pharmacology; Arabidopsis; Arabidopsis - genetics; Arabidopsis - physiology; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Calcineurin; calcium; Calcium-Binding Proteins - genetics; Calcium-Binding Proteins - metabolism; drought; Drought Resistance; Droughts; Gas exchange; Gene Expression Regulation, Plant; Germplasm; Guard cells; Kinases; Phenotypes; Plant Stomata - physiology; Plants, Genetically Modified; plasma membrane; Protein Serine-Threonine Kinases - genetics; Protein Serine-Threonine Kinases - metabolism; Solanum lycopersicum; Solanum lycopersicum - genetics; Solanum lycopersicum - metabolism; Solanum lycopersicum - physiology; Stomata; Tomatoes; Transpiration; Water loss
• ISSN: 0140-7791; 1365-3040
• DOI: 10.1111/pce.14887

Stomatal movement is critical for water transpiration, gas exchange, and responses to biotic stresses. Abscisic acid (ABA) induces stomatal closure to prevent water loss during drought. We report that Arabidopsis CIPK8 negatively regulates ABA‐mediated stomatal closure and drought tolerance. CIPK8 is highly enriched in guard cells and transcriptionally induced by ABA. Functional loss of CIPK8 results in hypersensitive stomatal closure to ABA and enhanced drought tolerance. Guard cell‐specific downregulation of CIPK8 mimics the phenotype of cipk8 whereas guard cell‐specific expression of a constitutive active CIPK8 (CIPK8CA) has an opposite effect, suggesting a cell autonomous activity of CIPK8. CIPK8 physically interacts with CBL1 and CBL9. Functional loss of CBL1 and CBL9 mimics ABA‐hypersensitive stomatal closure of cipk8 whereas abolishes the effect of CIPK8CA, indicating that CIPK8 and CBL1/CBL9 form a genetic module in ABA‐responsive stomatal movement. SlCIPK7, the functional homolog of CIPK8 in tomato (Solanum lycopersicum), plays a similar role in ABA‐responsive stomatal movement. Genomic editing of SlCIPK7 results in more drought‐tolerant tomato, making it a good candidate for germplasm improvement. Summary statement ABA‐induced stomatal closure is an important response of plants when challenged by drought. This study reports that Arabidopsis CIPK8 and its homolog SlCIPK7 in tomato negatively mediate stomatal closure upon ABA. Knocking‐out either gene results in more drought‐tolerant plants, making it a good candidate for germplasm improvement.