Structure of a polysaccharide MDP2-1 from Melastoma dodecandrum Lour. and its anti-inflammatory effects

• Published in: International journal of biological macromolecules 2024-04, Vol.265 (Pt 2), p.131015-131015, Article 131015
• Main Authors: Tang, Youying, Zhou, Mingyuan, Mao, Zian, Zhu, Bingqi, Zhou, Fangmei, Ye, Xiaoqing, Chen, Yuchi, Ding, Zhishan
• Format: Article
• Language: English
• Subjects: Anti-inflammation; Melastoma dodecandrum Lour; Polysaccharide
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.131015

The anti-inflammatory activity of polysaccharides derived from Melastoma dodecandrum Lour. was evaluated in pyretic mice and HEK-Blue™ hTLR4 cells. The testing led to the identification of MDP2-1, which was then investigated for its structural characteristics and anti-inflammatory effects. Results showed that MDP2-1 had a molecular weight of 29.234 kDa and primarily consisted of galactose, arabinose, rhamnose, glucose, glucuronic acid, and galacturonic acid. Its main backbone was composed of →4)-α-D-GalpA-(1→, →2)-α-L-Rhap-(1→, →3,4)-α-D-GalpA-(1→, →2,4)-α-D-GlcpA-(1→, and its side chains were connected by →4)-α-D-Galp-(1→, α-D-Galp-(1→, →4)-β-D-Glcp-(1→, and α-L-Araf-(1→. In vivo experiments on mice demonstrated that MDP2-1 attenuated LPS-induced acute lung injury, and in vitro experiments on RAW264.7 cells showed that MDP2-1 reduced the levels of inflammatory mediators and mitigated LPS-induced inflammatory damage by inhibiting the activation of the TLR4 downstream NF-κB/MAPK pathway. These findings suggest that MDP2-1 is a novel anti-inflammatory agent for therapeutic interventions. [Display omitted] •A polysaccharide MDP2-1 was isolated from the ethnic medicine Melastoma dodecandrum Lour. with anti-inflammatory function.•The structural characteristics and biological activity of MDP2-1 were studied.•Atomization of MDP2-1 can attenuate ALI induced by LPS in mice.•The anti-inflammatory effect of MDP2-1 is realized through the NF-κB/MAPK signaling pathway downstream of TLR4.