Monitoring of chicken houses and an attached egg-processing facility in a laying farm for Salmonella contamination between 1994 and 1998

• Published in: Journal of food protection 2001-12, Vol.64 (12), p.1912-1916
• Main Authors: MURASE, Toshiyuki, SENJYU, Kazuko, MAEDA, Takeshi, TANAKA, Masayuki, SAKAE, Hiroshi, MATSUMOTO, Yohji, KANEDA, Yoshiko, ITO, Toshihiro, OTSUKI, Koichi
• Format: Article
• Language: English
• Subjects: Animal Husbandry - methods; Animals; Biological and medical sciences; Chickens; Egg and egg product industries; Eggs - microbiology; Electrophoresis, Gel, Pulsed-Field - veterinary; Female; Food Contamination; Food industries; Fundamental and applied biological sciences. Psychology; Housing, Animal; Poultry Diseases - epidemiology; Poultry Diseases - microbiology; Poultry Diseases - transmission; Salmonella - classification; Salmonella - isolation & purification; Salmonella Infections, Animal - epidemiology; Salmonella Infections, Animal - microbiology; Salmonella Infections, Animal - transmission; Serotyping
• ISSN: 0362-028X; 1944-9097
• DOI: 10.4315/0362-028x-64.12.1912

Two chicken houses and an attached egg-processing facility in a laying farm were sampled between 1994 and 1998 to investigate Salmonella contamination. Each of the houses was environmentally controlled and fitted with egg belts that transported eggs from the houses to the egg-processing facility. Four hundred twenty-eight Salmonella isolates were obtained from 904 environmental samples collected from the houses. Two hundred fifty-two of the 428 (58.9%) isolates yielded five serotypes as follows: Salmonella enterica subsp. enterica serovar Livingstone, Salmonella serovar Cerro, Salmonella serovar Montevideo, Salmonella serovar Mbandaka, and Salmonella serovar Corvallis. The remaining (41.1%, 176 of 428) isolates included four other serotypes and isolates that were untypeable. Salmonella isolates obtained from the drain water collected after the washing of the eggs in the egg-processing facility yielded the same serotypes as those found in the chicken houses. Strains having an identical pulsed-field gel electrophoresis (PFGE) pattern were continually recovered from a house for more than 1 year. Several strains of Salmonella Cerro, Salmonella Mbandaka, and Salmonella Montevideo obtained from both the houses and from the egg-processing facility were indistinguishable by PFGE, respectively. These results suggest that Salmonella organisms originating from a single clone colonized the chicken houses and that the egg belts are likely to be one of the means by which Salmonella organisms are spread from one house to the others.