Immobilization of lysozyme on polyvinylalcohol films for active packaging applications

A new technique for the immobilization of lysozyme onto the surface of polyvinylalcohol films is presented. The active compound was sprayed along with a suitable bonding agent onto the surface of the cross-linked polymeric matrix. Active compound release tests determined the amount of lysozyme immob...

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Bibliographic Details
Published in:Journal of food protection 2006-04, Vol.69 (4), p.866-870
Main Authors: Conte, A, Buonocore, G.G, Bevilacqua, A, Sinigaglia, M, Del Nobile, M.A
Format: Article
Language:English
Subjects:
Anti-Infective Agents - pharmacology
antimicrobial properties
Biological and medical sciences
coatings
Colony Count, Microbial
Consumer Product Safety
crosslinking
Drug Packaging - methods
Enzymes, Immobilized
Food industries
Food Microbiology
food packaging
Food Packaging - methods
Food Preservation - methods
Fundamental and applied biological sciences. Psychology
General aspects
Handling, storage, packaging, transport
Humans
immobilized enzymes
lysozyme
Microbial Sensitivity Tests
Micrococcus - drug effects
Micrococcus - growth & development
Micrococcus luteus
Micrococcus lysodeikticus
Muramidase - pharmacology
natural additives
packaging materials
plastic film
poly(vinyl alcohol)
Polyvinyl Alcohol
surfaces
Time Factors
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Summary:A new technique for the immobilization of lysozyme onto the surface of polyvinylalcohol films is presented. The active compound was sprayed along with a suitable bonding agent onto the surface of the cross-linked polymeric matrix. Active compound release tests determined the amount of lysozyme immobilized on the film surface. With the use of Micrococcus lysodeikticus, the antimicrobial activity of the films was determined and the results correlated with the amount of immobilized lysozyme. This new technique was effective for immobilizing the enzyme, and the developed films were active against the test microorganism. Results were compared with those obtained with a different immobilizing technique, in which the active compound was bound into the bulk of the polymeric film. As expected, the surface-immobilized lysozyme films have a higher antimicrobial activity than bulk-bound films.
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028x-69.4.866