Biofunctionalization of gold nanoparticles and their spectral properties

A simple chemical procedure for fixing streptavidin molecules on the periphery of commercial Au nanoparticles stabilized by citrate groups is presented. Using a flocculation test, we determine the adequate quantity of streptavidin molecules necessary for coating Au nanoparticles with approximately o...

Full description

Saved in:
Bibliographic Details
Published in:Microelectronic engineering 2007-05, Vol.84 (5), p.1710-1713
Main Authors: Geneviève, Mike, Vieu, Christophe, Carles, Robert, Zwick, Antoine, Brière, Guillaume, Salomé, Laurence, Trévisiol, Emmanuelle
Format: Article
Language:English
Subjects:
Applied sciences
Biofunctionalization
Electronics
Exact sciences and technology
Gold nanoparticles
Microelectronic fabrication (materials and surfaces technology)
Photoluminescence
Raman spectroscopy
Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices
Streptavidin
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A simple chemical procedure for fixing streptavidin molecules on the periphery of commercial Au nanoparticles stabilized by citrate groups is presented. Using a flocculation test, we determine the adequate quantity of streptavidin molecules necessary for coating Au nanoparticles with approximately one monolayer of streptavidin. Our results indicate that on average, one molecule of streptavidin occupies an area of 40 nm 2 on the surface of the Au nanoparticles, which is the good order of magnitude with respect to the size of this biomolecule. The optical properties of these nanoparticles were found to be sensitive to the biofunctionalization process. We have observed that the changes in photoluminescence spectra could be used to monitor the coverage ratio of the nanoparticles with streptavidin.
ISSN:0167-9317
1873-5568
DOI:10.1016/j.mee.2007.01.247