A wireless magnetoelastic biosensor for the direct detection of organophosphorus pesticides

An organophosphorus (OP) pesticide sensor was fabricated by applying a pH-sensitive polymer coating and organophosphorus hydrolase (OPH) enzyme onto the surface of a magnetoelastic sensor, the magnetic analogue of the better-known surface acoustic wave sensor. Organophosphorus hydrolase catalyses th...

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Bibliographic Details
Published in:Analyst (London) 2007-04, Vol.132 (4), p.338-343
Main Authors: ZOUROB, Mohammed, ONG, Keat G, KEFENG ZENG, MOUFFOUK, Fouzi, GRIMES, Craig A
Format: Article
Language:English
Subjects:
Analytical chemistry
Aryldialkylphosphatase - chemistry
Biological and medical sciences
Biosensing Techniques
Biosensors
Biotechnology
Chemistry
Elasticity
Environmental Monitoring - instrumentation
Environmental Monitoring - methods
Environmental Pollutants - analysis
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Gels
General, instrumentation
Magnetics
Methods. Procedures. Technologies
Organophosphorus Compounds - analysis
Pesticides - analysis
Various methods and equipments
Viscosity
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Summary:An organophosphorus (OP) pesticide sensor was fabricated by applying a pH-sensitive polymer coating and organophosphorus hydrolase (OPH) enzyme onto the surface of a magnetoelastic sensor, the magnetic analogue of the better-known surface acoustic wave sensor. Organophosphorus hydrolase catalyses the hydrolysis of a wide range of organophosphorus compounds, which changes the pH in the hydrogel. This article describes the application of the magnetoelastic sensor for the detection of OP pesticides by measuring the changes in viscoelasticity caused by the swelling/shrinking of the pH-responsive polymer when exposed to the pesticides. The sensor was successfully used to detect paraoxon and parathion down to a concentration of 1 x 10(-7) and 8.5 x 10(-7) M respectively.
ISSN:0003-2654
1364-5528
DOI:10.1039/b616035b