Rapid Method for Biogenic Amines Evaluation in Fish Meal

A novel enzyme-based colorimetric method for hista-mine determination in fish meal was developed. It is based upon hista-mine oxidation by diamine oxidase and detection of the hydrogen peroxide generated using a peroxidase catalyzed reaction coupled with phenol-aminoantipyrine. At optimum assay cond...

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Bibliographic Details
Published in:Journal of aquatic food product technology 1999-12, Vol.8 (4), p.71-83
Main Authors: Albrecht-Ruiz, Miguel, Clark-Leza, Daniel, Aleman-Polo, Miguel
Format: Article
Language:English
Subjects:
Biogenic amines
diamine oxidase
fish meal
histamine
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Summary:A novel enzyme-based colorimetric method for hista-mine determination in fish meal was developed. It is based upon hista-mine oxidation by diamine oxidase and detection of the hydrogen peroxide generated using a peroxidase catalyzed reaction coupled with phenol-aminoantipyrine. At optimum assay conditions (temperature: 50°C, pH: 9.0), this technique allowed the accurate quantification of histamine + putrescine + cadaverine levels (amine index) between 200 to 2000 ppm. The method represents a good alternative for the rapid detection of potentially toxic fish meal.
ISSN:1049-8850
1547-0636
DOI:10.1300/J030v08n04_07