Production of quinolone derivatives in Escherichia coli

Alkyl-4-quinolones (AQs) are natural compounds synthesized by bacteria. Members of this group are known quorum-sensing molecules. Other biological functions, such as anti-bacterial, anti-algal, antifungal, and anti-malaria activities have also been reported. The synthetic pathways of AQs have been v...

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Bibliographic Details
Published in:Applied biological chemistry 2022-12, Vol.65 (1), p.65-65, Article 65
Main Authors: Park, Yeo-Jin, Choi, Gyu‑Sik, Lee, Shin-Won, Ahn, Joong-Hoon
Format: Article
Language:English
Subjects:
Algae
Applied Microbiology
Bacteria
Biological Techniques
Bioorganic Chemistry
Chemistry
Chemistry and Materials Science
Chromatography
Cloning
Copy number
E coli
Escherichia coli
Fungicides
Genes
Malaria
Microorganisms
Plasmids
Pseudomonas aeruginosa
Quinolones
quorum sensing
Substrates
Vector-borne diseases
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Summary:Alkyl-4-quinolones (AQs) are natural compounds synthesized by bacteria. Members of this group are known quorum-sensing molecules. Other biological functions, such as anti-bacterial, anti-algal, antifungal, and anti-malaria activities have also been reported. The synthetic pathways of AQs have been validated in Pseudomonas aeruginosa . Five genes ( pqsA–E ) are involved in the synthesis of 2-heptyl-4(1H)-quinolone (HHQ). To synthesize HHQ in a microbial system, pqsA–E genes were introduced into Escherichia coli and HHQ and 2-methyl-4(1H)-quinolone (MHQ) were synthesized. After the copy number, construct promoters, and substrate supplements were optimized, 141.3 mg/L MHQ and 242.8 mg/L HHQ were synthesized.
ISSN:2468-0834
2468-0842
DOI:10.1186/s13765-022-00733-7