Rapid identification,fluconazole and micafungin susceptibility testing of Candida species from blood culture by a short incubation method

This study aimed to develop and validate a rapid method for identification by MALDI-TOF system and determination of the susceptibility to Fluconazole and Micafungin by broth microdilution among Candidaspecies causing bloodstream infections. Subcultures from blood culture bottles were incubated for 5...

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Published in:Diagnostic microbiology and infectious disease 2024-06, Vol.109 (2), p.116271-116271, Article 116271
Main Authors: de Souza, Ândrea Celestino, Pereira, Dariane Castro, Barth, Patricia Orlandi, Roesch, Eliane Würdig, Lutz, Larissa, Aquino, Valério Rodrigues, Goldani, Luciano Zubaran
Format: Article
Language:English
Subjects:
Antifungal Agents - pharmacology
Blood culture
Blood Culture - methods
Candida
Candida - classification
Candida - drug effects
Candidemia - diagnosis
Candidemia - microbiology
Echinocandins - pharmacology
Fluconazole - pharmacology
Humans
Lipopeptides - pharmacology
MALDI-TOF MS
Micafungin - pharmacology
Microbial Sensitivity Tests - methods
Microdilution
Rapid diagnosis
Reproducibility of Results
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Time Factors
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Summary:This study aimed to develop and validate a rapid method for identification by MALDI-TOF system and determination of the susceptibility to Fluconazole and Micafungin by broth microdilution among Candidaspecies causing bloodstream infections. Subcultures from blood culture bottles were incubated for 5 hours (+/- 1h) and used to perform the tests, so that the turnaround time of rapid identification and susceptibility profile was about 5 and 24 hours, respectively. The rapid identification showed agreement of 92.05 %. Regarding the rapid broth microdilution for Fluconazole and Micafungin, the agreement was 97.06 % (p
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2024.116271