Immobilization of the horseradish peroxide on the mult-wall nanotube and its application for H2O2 sensing

In order to immobilize enzyme to multi-wall nanotube (MWNT), the MWNT was coated by the electrochemical polymerization of aniline. The horseradish peroxide (HRP) was immobilized on the surface of the polyaniline (PANI)-coated MWNT (PANI-MWNT film) by using gold nanoparticle (physical immobilization)...

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Bibliographic Details
Published in:Key engineering materials 2007-01, Vol.342-343, p.869-872
Main Authors: Seo, Kang-Deuk, Oh, Seong-Dae, Choi, Seong-Ho, Oh, Sang-Hyub, Woo, Jin-Chun, Kim, Sang-Ho
Format: Article
Language:English
Online Access:Get full text
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Summary:In order to immobilize enzyme to multi-wall nanotube (MWNT), the MWNT was coated by the electrochemical polymerization of aniline. The horseradish peroxide (HRP) was immobilized on the surface of the polyaniline (PANI)-coated MWNT (PANI-MWNT film) by using gold nanoparticle (physical immobilization). The HRP was also immobilized on the surface of PANIMWNT film by using 2-aminothiophenol and Au nanoparticle (self-assembling immobilization). HRP was immobilized on the surface of PANI-MWNT electrode by using glutaric dialdehyde (covalent immobilization). The sensing efficiency of HRP-MWNT electrodes for H2O2 was evaluated by amperometric method. The sensing efficiency of HRP-MWNT electrodes for H2O2 was as following order; physical immobilization > covalent immobilization > self-assembling immobilization.
ISSN:1013-9826
DOI:10.4028/0-87849-436-7.869