Evidence on the role of protein biosynthesis in the induction of heat tolerance of Lactobacillus rhamnosus GG by pressure pre-treatment

It was the aim of this work to evaluate, whether and to which extent heat resistance of Lactobacillus rhamnosus GG is affected by mild pressure treatments prior to exposure to lethal temperatures, such as during spray-drying. It was observed that cells pressure pre-treated at 100 MPa at 37 °C for 10...

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Bibliographic Details
Published in:International journal of food microbiology 2004-11, Vol.96 (3), p.307-313
Main Authors: Ananta, E., Knorr, D.
Format: Article
Language:English
Subjects:
Bacterial Proteins - biosynthesis
Bacterial Proteins - drug effects
Biological and medical sciences
Cell Membrane
Chloramphenicol - pharmacology
Flow Cytometry
Food industries
Food Microbiology
Fundamental and applied biological sciences. Psychology
heat shock proteins
heat stress
Heat tolerance
heat treatment
High hydrostatic pressure
high pressure treatment
Hot Temperature
Hydrostatic Pressure
induced resistance
lactic acid bacteria
Lactobacillus - metabolism
Lactobacillus rhamnosus
pretreatment
Probiotic bacteria
Probiotics
protein synthesis
protein synthesis inhibitors
Protein Synthesis Inhibitors - pharmacology
temperature
Time Factors
viability
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Summary:It was the aim of this work to evaluate, whether and to which extent heat resistance of Lactobacillus rhamnosus GG is affected by mild pressure treatments prior to exposure to lethal temperatures, such as during spray-drying. It was observed that cells pressure pre-treated at 100 MPa at 37 °C for 10 min showed higher survival than untreated cells when exposed to heat challenge at 60 °C. To gain more insights on the cellular mode of action of pressure induced heat tolerance, flow cytometric analysis was applied in combination with functional dye LIVE/DEAD®BacLight™ bacterial viability kit. Dot plot analysis showed that a lower degree of membrane damage was observed at pressure pre-treated cells upon heat treatment at 60 °C for 3 min. Evaluation of heat inactivation kinetics of cells pressure treated in the presence of chloramphenicol, a protein synthesis inhibitor, pointed out the potential contribution of pressure-induced protein biosynthesis in the enhancement of bacterial heat tolerance.
ISSN:0168-1605
1879-3460
DOI:10.1016/j.ijfoodmicro.2004.04.012