Signal Amplification in Immunoassays Using Labeling via Boronic Acid Binding to the Sugar Moiety of Immunoglobulin G: Proof of Concept for Glycated Hemoglobin

A novel electrochemical immunoassay based on the multiple affinity labeling of the indicator antibody with an electro-active tag is presented. The concept is illustrated for the determination of the glycated hemoglobin HbA1c in hemoglobin samples. Hemoglobin is adsorbed to the surfactant-modified su...

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Bibliographic Details
Published in:Analytical letters 2007-01, Vol.40 (7), p.1434-1444
Main Authors: Halámek, J., Wollenberger, U., Stöcklein, W. F. M., Warsinke, A., Scheller, F. W.
Format: Article
Language:English
Subjects:
Analytical chemistry
boronic acid
Chemistry
Electrochemical methods
Exact sciences and technology
ferrocene
General, instrumentation
hemoglobin
Immunoassay
Miscellaneous
QCN
voltammetry
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Summary:A novel electrochemical immunoassay based on the multiple affinity labeling of the indicator antibody with an electro-active tag is presented. The concept is illustrated for the determination of the glycated hemoglobin HbA1c in hemoglobin samples. Hemoglobin is adsorbed to the surfactant-modified surface of a piezoelectric quartz crystal. Whereas the quartz crystal nanobalance is used to validate the total Hb binding, the HbA1c on the sensor surface is recognized by an antibody and quantified electrochemically after the sugar moieties of the antibody have been labeled in-situ with ferroceneboronic acid. The sensitivity of this sensor is about threefold higher than the sensitivity of a hemoglobin sensor, where the ferroceneboronic acid is bound directly to HbA1c.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032710701327096