Extended period of ventilation before delayed cord clamping augments left‐to‐right shunting and decreases systemic perfusion at birth in preterm lambs

• Published in: The Journal of physiology 2024-04, Vol.602 (8), p.1791-1813
• Main Authors: Smolich, Joseph J., Kenna, Kelly R., Mynard, Jonathan P.
• Format: Article
• Language: English
• Subjects: Birth; birth transition; Blood flow; delayed cord clamping; ductus arteriosus; Fetuses; foramen ovale; Heart; left‐to‐right shunting; Perfusion; pulmonary blood flow; systemic arterial perfusion; Ventilation; Ventricle
• ISSN: 0022-3751; 1469-7793
• DOI: 10.1113/JP285799

Previous studies have suggested that an extended period of ventilation before delayed cord clamping (DCC) augments birth‐related rises in pulmonary arterial (PA) blood flow. However, it is unknown whether this greater rise in PA flow is accompanied by increases in left ventricular (LV) output and systemic arterial perfusion or whether it reflects enhanced left‐to‐right shunting across the ductus arteriosus and/or foramen ovale (FO), with decreased systemic arterial perfusion. Using an established preterm lamb birth transition model, this study compared the effect of a short (∼40 s, n = 11), moderate (∼2 min, n = 11) or extended (∼5 min, n = 12) period of initial mechanical lung ventilation before DCC on flow probe‐derived perinatal changes in PA flow, LV output, total systemic arterial blood flow, ductal shunting and FO shunting. The LV output was relatively stable during initial ventilation but increased after DCC, with similar responses in all groups. Systemic arterial flow patterns displayed only minor differences during brief and moderate periods of initial ventilation and were similar after DCC. However, an increase in PA flow was augmented with an extended initial ventilation (P < 0.001), owing to an earlier onset of left‐to‐right ductal and FO shunting (P < 0.001), and was accompanied by a pronounced reduction in total systemic arterial flow (P = 0.005) that persisted for 4 min after DCC (P ≤ 0.039). These findings suggest that, owing to increased left‐to‐right shunting and a greater reduction in systemic arterial perfusion, an extended period of ventilation before DCC does not result in greater perinatal circulatory benefits than shorter periods of initial ventilation in the birth transition. Key points Previous studies suggest that an extended period of initial ventilation before delayed cord clamping (DCC) augments birth‐related rises in pulmonary arterial (PA) blood flow. It is unknown whether this greater rise in PA flow is accompanied by an increased left ventricular output and systemic arterial perfusion or whether it reflects enhanced left‐to‐right shunting across the ductus arteriosus and/or foramen ovale, with decreased systemic arterial perfusion. Anaesthetized preterm fetal lambs instrumented with central arterial flow probes underwent a brief (∼40 s), moderate (∼2 min) or extended (∼5 min) period of ventilation before DCC. Perinatal changes in left ventricular output were similar in all groups, but extended initial ventilation augmented