Efficient Whole-Cell Biocatalytic Synthesis of N-Acetyl-D-neuraminic Acid

N‐Acetyl‐D‐neuraminic acid (Neu5Ac) was efficiently synthesized from lactate and a mixture of N‐acetyl‐D‐glucosamine (GlcNAc) and N‐acetyl‐D‐mannosamine (ManNAc) by whole cells. The biotransformation utilized Escherichia coli cells (Neu5Ac aldolase), Pseudomonas stutzeri cells (lactate oxidase compo...

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Bibliographic Details
Published in:Advanced synthesis & catalysis 2007-07, Vol.349 (10), p.1614-1618
Main Authors: Xu, Ping, Qiu, Jian Hua, Zhang, Yi Nan, Chen, Jing, Wang, Peng George, Yan, Bing, Song, Jing, Xi, Ri Mo, Deng, Zi Xin, Ma, Cui Qing
Format: Article
Language:English
Subjects:
biotransformation
lactate oxidase component
N-acetyl-D-neuraminic acid (Neu5Ac)
Neu5Ac aldolase
sialic acids
whole cells
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Summary:N‐Acetyl‐D‐neuraminic acid (Neu5Ac) was efficiently synthesized from lactate and a mixture of N‐acetyl‐D‐glucosamine (GlcNAc) and N‐acetyl‐D‐mannosamine (ManNAc) by whole cells. The biotransformation utilized Escherichia coli cells (Neu5Ac aldolase), Pseudomonas stutzeri cells (lactate oxidase components), GlcNAc/ManNAc and lactate. By this process, 18.32±0.56 g/liter Neu5Ac were obtained from 65.61±2.70 g/liter lactate as an initial substrate input. Neu5Ac (98.4±0.4 % purity, 80.87±0.79 % recovery yield) was purified by anionic exchange chromatography. Our results demonstrate that the reported Neu5Ac biosynthetic process can compare favorably with natural product extraction or chemical synthesis processes.
ISSN:1615-4150
1615-4169
DOI:10.1002/adsc.200700094