Application of a new zwitterionic hydrophilic interaction chromatography column for determination of paralytic shellfish poisoning toxins

A novel method for paralytic shellfish poisoning (PSP) toxins which is based on the chromatographic separation of the toxins using a zwitterionic (ZIC) hydrophilic interaction chromatography (HILIC) column is presented. Efficient retention of the polar PSP toxins on the ZIC‐HILIC column allowed thei...

Full description

Saved in:
Bibliographic Details
Published in:Journal of separation science 2007-08, Vol.30 (12), p.1821-1826
Main Authors: Diener, Marc, Erler, Katrin, Christian, Bernd, Luckas, Bernd
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Chromatography - methods
Chromatography, Liquid - methods
Fish and seafood industries
Food industries
Food toxicology
Fundamental and applied biological sciences. Psychology
Hydrophilic interaction chromatography
Ions
LC-MS/MS
Marine Toxins - analysis
Marine Toxins - isolation & purification
Mass Spectrometry
Models, Chemical
Molecular Conformation
Paralytic shellfish poisoning
Phytoplankton - metabolism
Reproducibility of Results
Saxitoxin - analysis
Saxitoxin - isolation & purification
Shellfish
Spectrometry, Fluorescence
Time Factors
Zwitterionic-HILIC column
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A novel method for paralytic shellfish poisoning (PSP) toxins which is based on the chromatographic separation of the toxins using a zwitterionic (ZIC) hydrophilic interaction chromatography (HILIC) column is presented. Efficient retention of the polar PSP toxins on the ZIC‐HILIC column allowed their selective and sensitive determination by the application of mass spectrometric (MS/MS) detection or as derivatives after oxidation prior to fluorescence detection (FD). Low buffer concentrations and the omission of ion‐pair reagents decreased the limits of detection (LODs) by MS/MS analysis and showed a good linearity for both methods of detection. This method can be applied for the qualitative and quantitative determination of PSP toxins in various types of phytoplankton, and for the routine analysis of seafood.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200700025