Amperometric Glucose Determination by Means of Glucose Oxidase Immobilized on a Cellulose Acetate Film: Dependence on the Immobilization Procedures

Glucose microelectrodes were prepared by immobilizing glucose oxidase onto a cellulose acetate film coating a platinum wire. Hexamethylenediamine (HMDA) and Glutaraldehyde (GA) were employed as spacer and coupling agent, respectively. Sensitivities and linear response ranges were studied as a functi...

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Bibliographic Details
Published in:Electroanalysis (New York, N.Y.) N.Y.), 2007-09, Vol.19 (17), p.1787-1793
Main Authors: Portaccio, Marianna, Durante, Daniela, Viggiano, Andrea, Di Martino, Silvana, De Luca, Paola, Di Tuoro, Daniela, Bencivenga, Umberto, Rossi, Sergio, Canciglia, Paolo, De Luca, Bruno, Mita, Damiano Gustavo
Format: Article
Language:English
Subjects:
Cellulose acetate
Enzyme immobilization
Glucose biosensor
Glucose determination
Glucose oxidase
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Summary:Glucose microelectrodes were prepared by immobilizing glucose oxidase onto a cellulose acetate film coating a platinum wire. Hexamethylenediamine (HMDA) and Glutaraldehyde (GA) were employed as spacer and coupling agent, respectively. Sensitivities and linear response ranges were studied as a function of the relative amounts of HMDA and GA. The best sensitivity was found when HMDA and GA were 5% and 2.5% in aqueous solutions, respectively. Taking as a reference the functioning of this biosensor, the roles of HMDA and GA percentages appear to be opposed when the extension of the linear response range is considered. Indeed, an increase of one unit in HMDA percentage (from 5 to 6 %) induces an increase in the extension of the linear response range equal to that obtained with a decrease of one unit of GA percentage (from 2.5 to 1.5%).
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.200703934