fast CE method for the achiral separation of methadone and its major metabolites, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine and 2-ethyl-5-methyl-3,3-diphenyl-1-pyrroline

• Published in: Electrophoresis 2007-10, Vol.28 (19), p.3566-3569
• Main Authors: Kelly, Tamsin, Doble, Philip, Dawson, Michael
• Format: Article
• Language: English
• Subjects: 2-Ethyl-5-methyl-3; 2-Ethylidene-1; 2‐Ethylidene‐1,5‐dimethyl‐3,3‐diphenylpyrrolidine; 2‐Ethyl‐5‐methyl‐3,3‐diphenyl‐1‐pyrroline; 3-diphenyl-1-pyrroline; 3-diphenylpyrrolidine; 5-dimethyl-3; Calibration; Coated Materials, Biocompatible - chemistry; Dextran Sulfate - chemistry; Dynamic doubly coated capillaries; Electrophoresis, Capillary - instrumentation; Electrophoresis, Capillary - methods; Forensic Toxicology - methods; Hydrogen-Ion Concentration; Methadone; Methadone - analysis; Methadone - metabolism; Phosphates; Poly(diallyldimethylammonium chloride); Polyethylenes - chemistry; Pyrrolidines - analysis; Pyrrolidines - metabolism; Quaternary Ammonium Compounds - chemistry; Reproducibility of Results; Sensitivity and Specificity; Spectrophotometry, Ultraviolet - methods
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/elps.200700090

The utilization of dynamic doubly coated capillaries for a fast separation of methadone and its two major metabolites, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl-3,3-diphenyl-1-pyrroline (EMDP) was investigated. The coated capillaries were prepared using a polycation of poly(diallyldimethylammonium chloride) and a polyanion of dextran sulfate. A fast achiral separation was developed using the coated capillaries with a BGE of 100 mM phosphate buffer at pH 2.6. Complete achiral separation of methadone, EDDP and EMDP was achieved, with migration times of approximately 4 min. The method offers considerable advantages with respect to BGE simplicity and analysis time compared to previously published CE methods for methadone and its related analytes.