Preparation and biodistribution of [67Ga]-DTPA-rituximab in normal rats

Rituximab was successively labeled with [Ga]-gallium chloride after residulation with freshly prepared cyclic DTPA-dianhydride. The best results of the conjugation were obtained by the addition of 1 ml of a rituximab pharmaceutical solution (5 mg/ml, in phosphate buffer, pH 8) to a glass tube pre-co...

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Bibliographic Details
Published in:Journal of radioanalytical and nuclear chemistry 2007-10, Vol.274 (1), p.175-179
Main Authors: Jalilian, A. R., Mirsadeghi, L., Haji-Hosseini, R.
Format: Article
Language:English
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Summary:Rituximab was successively labeled with [Ga]-gallium chloride after residulation with freshly prepared cyclic DTPA-dianhydride. The best results of the conjugation were obtained by the addition of 1 ml of a rituximab pharmaceutical solution (5 mg/ml, in phosphate buffer, pH 8) to a glass tube pre-coated with DTPA-dianhydride (0.01 mg) at 25 deg C with continuous mild stirring for 30 minutes. Radio thin-layer chromatography showed an overall radiochemical purity of 96-99% at optimized conditions (specific activity = 300-500 MBq/mg, labeling efficiency 77%). The final isotonic Ga-DTPA-rituximab complex was checked by gel electrophoresis for radiolysis control. Radio-TLC was performed to ensure the formation of only one species, followed by filtration through a 0.22 mum filter. Preliminary in vivo studies in normal rat model was performed to determine the biodistribution of the radioimmunoconjugate up to 6 hours.
ISSN:0236-5731
1588-2780
DOI:10.1007/s10967-006-6906-0