Gut microbiota dynamics in KK-Ay mice: restoration following antibiotic treatment

• Published in: Folia microbiologica 2024-10, Vol.69 (5), p.1159-1173
• Main Authors: Hong, Jinni, Fu, Tingting, Liu, Weizhen, Yu, Miao, Lin, Yanshan, Min, Cunyun, Lin, Datao
• Format: Article
• Language: English
• Subjects: Abundance; Animals; Anti-Bacterial Agents - administration & dosage; Anti-Bacterial Agents - pharmacology; Antibiotics; Applied Microbiology; Bacteria; Bacteria - classification; Bacteria - drug effects; Bacteria - genetics; Bacteria - isolation & purification; Biodiversity; Biomedical and Life Sciences; class; Comparative analysis; Composition; DNA; DNA sequencing; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Dynamic response; Environmental Engineering/Biotechnology; family; Feces - microbiology; Gastrointestinal Microbiome - drug effects; Gene sequencing; Immunology; Intestinal microflora; intestinal microorganisms; Lachnospiraceae; Life Sciences; Male; Mice; microbial communities; Microbiology; Microbiomes; Microbiota; Microorganisms; Original Article; Phylogeny; Renal function; Restoration; RNA, Ribosomal, 16S - genetics; rRNA 16S; Ruminococcaceae; Sequence analysis; Sequence Analysis, DNA
• ISSN: 0015-5632; 1874-9356; 1874-9356
• DOI: 10.1007/s12223-024-01157-0

The primary aim of this study was to investigate the alterations in the microbial community of KK-Ay mice following antibiotic treatment. A comparative analysis of the gut microbiota was conducted between KK-Ay mice treated with antibiotics and those without treatment. The microbial community dynamics in antibiotic-treated KK-Ay mice were meticulously assessed over an eight-week period using 16S rDNA sequencing analysis. Simultaneously, dynamic renal function measurements were performed. The results demonstrated a marked decrease in bacterial DNA abundance following antibiotic intervention, coupled with a substantial reduction in bacterial diversity and a profound alteration in microbial composition. These observed microbiota changes persisted in the KK-Ay mice throughout the eight-week post-antibiotic treatment period. Particularly noteworthy was the reemergence of bacterial populations after two weeks or more, resulting in a microbiota composition resembling that of untreated KK-Ay mice. This transition was characterized by a significant increase in the abundance of clostridia at the class level, Lachnospirales and Oscillospirales at the order level, and Lachnospiraceae, Oscillospiraceae, and Ruminococcaceae at the family level. Concurrently, there was a notable decrease in Clostridia_UCG-014. The observed alterations in the gut microbiota of antibiotic-treated KK-Ay mice suggest a dynamic response to antibiotic intervention and subsequent restoration towards the original untreated state.