Altered Grooming Cycles in Transgenic Drosophila

• Published in: Behavior genetics 2024-05, Vol.54 (3), p.290-301
• Main Authors: Ringo, John M., Segal, Daniel
• Format: Article
• Language: English
• Subjects: Animals; Behavioral Science and Psychology; Cell interactions; Circuits; Clinical Psychology; Drosophila; Drosophila melanogaster - genetics; Drosophila Proteins - metabolism; Dynamins - genetics; Dynamins - metabolism; Editing; Genes; Grooming; Head; Health Psychology; Humans; Insects; Legs; Motor neurons; Neural networks; Neurons; Neurons - metabolism; Original Research; Psychology; Public Health; Recycling; RNA editing; RNA-mediated interference; Temperature effects
• ISSN: 0001-8244; 1573-3297
• DOI: 10.1007/s10519-024-10180-3

Head grooming in Drosophila consists of repeated sweeps of the legs across the head, comprising regular cycles. We used the GAL4-UAS system to study the effects of overexpressing shibire ts1 and of Adar knockdown via RNA interference, on the period of head-grooming cycles in Drosophila . Overexpressing shibire ts1 interferes with synaptic vesicle recycling and thus with cell communication, while Adar knockdown reduces RNA editing of neuronal transcripts for a large number of genes. All transgenic flies and their controls were tested at 22° to avoid temperature effects; in wild type, cycle frequency varied with temperature with a Q 10 of 1.3. Two experiments were performed with transgenic shibire ts1 : (1) each fly was heat-shocked for 10 min at 30° immediately before testing at 22° and (2) flies were not heat shocked. In both experiments, cycle period was increased when shibire ts1 was overexpressed in all neurons, but was not increased when shibire ts1 was overexpressed in motoneurons alone. We hypothesize that grooming cycles in flies overexpressing shibire ts1 are lengthened because of synaptic impairment in neural circuits that control head-grooming cycles. In flies with constitutive, pan-neuronal Adar knockdown, cycle period was more variable within individuals, but mean cycle period was not significantly altered. We conclude that RNA editing is essential for the maintenance of within-individual stereotypy of head-grooming cycles.