Inhibition of hyphal formation together with biochar addition promotes erythritol production by Yarrowia lipolytica

This study aimed to investigate the effect of hyphal formation in Yarrowia lipolytica and biochar addition on erythritol production by submerged fermentation. Hyphal formation significantly inhibited erythritol production by Y. lipolytica. Transcriptome analysis suggested that the impaired erythrito...

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Published in:Biotechnology and bioengineering 2024-06, Vol.121 (6), p.1937-1949
Main Authors: Liu, Fangmei, Xia, Kai, Chen, Yuqing, Zhu, Ling, Zhu, Lingzhi, Zhao, Xuequn, Sha, Ruyi, Huang, Jun
Format: Article
Language:English
Subjects:
Amino acids
Bagasse
Bioreactors
Bioreactors - microbiology
Cell walls
Charcoal
Charcoal - chemistry
Charcoal - pharmacology
Clonal deletion
Corn straw
Erythritol
Erythritol - biosynthesis
Erythritol - metabolism
Fermentation
Gene expression
Hyphae - drug effects
Hyphae - genetics
Hyphae - growth & development
Hyphae - metabolism
Lipid metabolism
Lipids
Metabolism
Sugarcane
Transcriptomes
Yarrowia - genetics
Yarrowia - metabolism
Yarrowia lipolytica
Yeasts
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Summary:This study aimed to investigate the effect of hyphal formation in Yarrowia lipolytica and biochar addition on erythritol production by submerged fermentation. Hyphal formation significantly inhibited erythritol production by Y. lipolytica. Transcriptome analysis suggested that the impaired erythritol synthesis of hyphal cells was associated with the differential expression of genes involved in amino acid metabolism, lipid metabolism, and cell wall stability. Deletion of RAS2 responsible for yeast-to-hypha transition and EYD1 included in erythritol degradation blocked hyphal formation and improved erythritol production. Biochar prepared from corncob, sugarcane bagasse (SB), corn straw, peanut shell, coconut shell, and walnut shell (WS) had a positive effect on erythritol production, of which WS pyrolyzed at 500°C (WSc) performed the best in flask fermentation. In a 3.7 L bioreactor, 220.20 ± 10 g/L erythritol with a productivity of 2.30 ± 0.10 g/L/h was obtained in the presence of 1.4% (w/v) WSc and 0.7% SBc (SB pyrolyzed at 500°C) within 96 h. These results suggest that inhibition of hyphal formation together with biochar addition is an efficient way to promote erythritol production.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.28704