Assessment of the efficiency and stability of enzymatic membrane reaction utilizing lipase covalently immobilized on a functionalized hybrid membrane

• Published in: Journal of biotechnology 2024-05, Vol.387, p.23-31
• Main Authors: Rezaie, Hosna, Kajani, Abolghasem Abbasi, Jafarian, Faranak, Asgari, Sina, Taheri-Kafrani, Asghar, Bordbar, Abdol-Khalegh
• Format: Article
• Language: English
• Subjects: Graphene oxide nanosheets; Immobilization; Lipase; Membrane; Polyethersulfone
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/j.jbiotec.2024.03.012

Enzyme immobilization in membrane bioreactors has been considered as a practical approach to enhance the stability, reusability, and efficiency of enzymes. In this particular study, a new type of hybrid membrane reactor was created through the phase inversion method, utilizing hybrid of graphene oxide nanosheets (GON) and polyether sulfone (PES) in order to covalently immobilize the Candida rugosa lipase (CRL). The surface of hybrid membrane was initially modified by (3-Aminopropyl) triethoxysilane (APTES), before the use of glutaraldehyde (GLU), as a linker, through the imine bonds. The resulted enzymatic hybrid membrane reactors (EHMRs) were then thoroughly analyzed by using field-emission scanning electron microscopy (FE-SEM), contact angle goniometry, surface free energy analysis, X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, attenuated total reflection (ATR), and energy-dispersive X-ray (EDX) spectroscopy. The study also looked into the impact of factors such as initial CRL concentration, storage conditions, and immobilization time on the EHMR's performance and activity, which were subsequently optimized. The results demonstrated that the CRLs covalently immobilized on the EHMRs displayed enhanced pH and thermal stability compared to those physically immobilized or free. These covalently immobilized CRLs could maintain over 60% of their activity even after 6 reaction cycles spanning 50 days. EHMRs are valuable biocatalysts in developing various industrial, environmental, and analytical processes. [Display omitted] •A new hybrid membrane reactor (HMR) was developed.•Candida rugosa lipase (CRL) was covalently immobilized on HMR.•Performance and activity of enzymatic hybrid membrane reactors (EHMRs) were optimized.•EHMRs maintain over 60% of their activity even after six reaction cycles spanning 50 days.