A multi-omics investigation of the lung injury induced by PM2.5 at environmental levels via the lung-gut axis

• Published in: The Science of the total environment 2024-05, Vol.926, p.172027-172027, Article 172027
• Main Authors: Dai, Shuiping, Wang, Zhenglu, Cai, Min, Guo, Tingting, Mao, Shengqiang, Yang, Ying
• Format: Article
• Language: English
• Subjects: China; chronic exposure; environment; gastrointestinal system; guanosine; Halomonas; Helicobacter; hypoxanthine; intestinal microorganisms; Lactobacillus; linoleic acid; Lung inflammation; Lung-gut axis; lungs; males; metabolism; metabolites; Metabolomes; Microbiomes; Multi-omics analysis; multiomics; Oscillospira; particulates; PM2.5; pulmonary emphysema; serotonin; spermidine; synapse; tryptophan
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2024.172027

Long-term exposure to fine particulate matter (PM2.5) posed injury for gastrointestinal and respiratory systems, ascribing with the lung-gut axis. However, the cross-talk mechanisms remain unclear. Here, we attempted to establish the response networks of lung-gut axis in mice exposed to PM2.5 at environmental levels. Male Balb/c mice were exposed to PM2.5 (dose of 0.1, 0.5, and 1.0 mg/kg) collected from Chengdu, China for 10 weeks, through intratracheally instillation, and examined the effect of PM2.5 on lung functions of mice. The changes of lung and gut microbiota and metabolic profiles of mice in different groups were determined. Furthermore, the results of multi-omics were conjointly analyzed to elucidate the primary microbes and the associated metabolites in lung and gut responsible for PM2.5 exposure. Accordingly, the cross-talk network and key pathways between lung-gut axis were established. The results indicated that exposed to PM2.5 0.1 mg/kg induced obvious inflammations in mice lung, while emphysema was observed at 1.0 mg/kg. The levels of metabolites guanosine, hypoxanthine, and hepoxilin B3 increased in the lung might contribute to lung inflammations in exposure groups. For microbiotas in lung, PM2.5 exposure significantly declined the proportions of Halomonas and Lactobacillus. Meanwhile, the metabolites in gut including L-tryptophan, serotonin, and spermidine were up-regulated in exposure groups, which were linked to the decreasing of Oscillospira and Helicobacter in gut. Via lung-gut axis, the activations of pathways including Tryptophan metabolism, ABC transporters, Serotonergic synapse, and Linoleic acid metabolism contributed to the cross-talk between lung and gut tissues of mice mediated by PM2.5. In summary, the microbes including Lactobacillus, Oscillospira, and Parabacteroides, and metabolites including hepoxilin B3, guanosine, hypoxanthine, L-tryptophan, and spermidine were the main drivers. In this lung-gut axis study, we elucidated some pro- and pre-biotics in lung and gut microenvironments contributed to the adverse effects on lung functions induced by PM2.5 exposure. [Display omitted] •The microbiota and metabolic profiles of lung and gut of mice after PM2.5 exposure were constructed.•The crosstalk between lung-gut axis of mice was revealed using multi-omics analysis.•PM2.5 induced a decline of probiotics, Halomonas and Lactobacillus in lung, and Oscillospira in gut.•Key metabolites involved in lung-gut axis were hepoxilin B3, g