Detection and comparison of neutrophil extracellular traps in tissue samples of peri‐implantitis, periodontitis, and healthy patients: A pilot study

Objective The aim of this study was to detect and compare the tissular expression of neutrophil extracellular traps (NETs) in peri‐implant and periodontal samples of patients with peri‐implantitis, periodontitis, and controls. Materials and Methods An observational study was performed on patients wi...

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Published in:Clinical implant dentistry and related research 2024-06, Vol.26 (3), p.631-641
Main Authors: Al‐Bakri, Sarmad Muayad Rasheed, Magan‐Fernandez, Antonio, Galindo‐Moreno, Pablo, O'Valle, Francisco, Martin‐Morales, Natividad, Padial‐Molina, Miguel, Mesa, Francisco
Format: Article
Language:English
Subjects:
Adult
Aged
Case-Control Studies
Citrulline
Connective tissue
Connective tissues
Electron microscopy
extracellular traps
Extracellular Traps - metabolism
Female
fluorescent antibody technique
Gum disease
Humans
Immunofluorescence
Immunohistochemistry
Leukocytes (neutrophilic)
Male
Microscopy
Middle Aged
Neutrophils
Neutrophils - pathology
Observational studies
Peri-Implantitis - metabolism
Peri-Implantitis - pathology
Periodontitis
Periodontitis - metabolism
Periodontitis - pathology
peri‐implantitis
Peroxidase
Pilot Projects
Teeth
Tissues
Transmission electron microscopy
Transplants & implants
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Summary:Objective The aim of this study was to detect and compare the tissular expression of neutrophil extracellular traps (NETs) in peri‐implant and periodontal samples of patients with peri‐implantitis, periodontitis, and controls. Materials and Methods An observational study was performed on patients with peri‐implantitis, periodontitis, and controls. Peri‐implant and/or periodontal clinical examinations were performed on each participant. Tissue samples were collected during tooth/implant extraction for clinical reasons. Electron microscopy analysis, Picro‐Sirius red staining, immunohistochemical (CD15), and immunofluorescence (citrullinated H3 and myeloperoxidase) techniques were performed to detect NET‐related structures and the degree of connective tissue destruction, between the study groups. Results Sixty‐four patients were included in the study: 28 peri‐implantitis, 26 periodontitis, and 10 controls, with a total of 51 implants, 26 periodontal teeth, and 10 control teeth. Neutrophil release of nuclear content was observed in transmission electron microscopy. Immunohistochemical analysis showed a greater CD15 expression in both peri‐implantitis and periodontitis compared to controls (p 
ISSN:1523-0899
1708-8208
1708-8208
DOI:10.1111/cid.13325