Using hemoglobin vesicles to treat operative hemorrhagic shock after pneu- monectomy in dog models: an experimental study

Hemoglobin vesicles (HbVs), considered as red blood cell substitutes, are liposomes encapsulating purified hemoglobin, with a phospholipid bilayer membrane (diameter: 250 nm; P50, 28 Torr). In this study, we aimed to investigate HbV function during hemorrhagic shock in lung resection and analyze the...

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Published in:Biomedical Research 2024/03/22, Vol.45(2), pp.91-101
Main Authors: NAKANO, Kei, KOHNO, Mitsutomo, ONOZAWA, Hiroto, HASHIMOTO, Ryo, OIWA, Kana, MASUDA, Ryota, YAMAGUCHI, Masatoshi, HATO, Tai, WATANABE, Masazumi, HORINOUCHI, Hirohisa, SAKAI, Hiromi, KOBAYASHI, Koichi, IWAZAKI, Masayuki
Format: Article
Language:English
Subjects:
Blood
Blood circulation
Blood pressure
Blood volume
Dogs
Erythrocytes
Hemoglobin
Hemorrhage
Hemorrhagic shock
Human serum albumin
Lungs
Mechanical ventilation
Oxygen
Phospholipids
Pneumonectomy
Respiratory function
Resuscitation
Serum albumin
Shock
Thoracic surgery
Thorax
Vesicles
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Summary:Hemoglobin vesicles (HbVs), considered as red blood cell substitutes, are liposomes encapsulating purified hemoglobin, with a phospholipid bilayer membrane (diameter: 250 nm; P50, 28 Torr). In this study, we aimed to investigate HbV function during hemorrhagic shock in lung resection and analyze the details of oxygen delivery. Left pneumonectomy was performed in dogs under mechanical ventilation, followed by rapid exsanguination of approximately 30% of the total circulating blood volume, which led to shock, reducing the mean arterial pressure (MAP) by approximately 60% of baseline. Subsequently, either 5% human serum albumin (HSA) or HbVs suspended in 5% HSA were infused for resuscitation. The MAP only recovered to 75% of baseline after HSA administration, but fully recovered (100%) after HbV administration, with significant differences between the groups (P < 0.005). Oxygen delivery was restored in the HbV group and was significantly higher than that in the HSA group (P < 0.0001). The infusion of HbVs dispersed in a 5% HSA solution compensated for the rapid loss of approximately 30% of the total circulating blood volume in a dog pneumonectomy model, even with impaired lung function. Thus, HbVs can be used for resuscitation from hemorrhagic shock during thoracic surgery.
ISSN:0388-6107
1880-313X
DOI:10.2220/biomedres.45.91