Dual function of magnetic nanocomposites-based SERS lateral flow strip for simultaneous detection of aflatoxin B1 and zearalenone

• Published in: Food chemistry 2024-07, Vol.446, p.138817-138817, Article 138817
• Main Authors: Yin, Limei, Cai, Jianrong, Ma, Lixin, You, Tianyan, Arslan, Muhammad, Jayan, Heera, Zou, Xiaobo, Gong, Yunyun
• Format: Article
• Language: English
• Subjects: Aflatoxin B1; Aflatoxin B1 - analysis; Fe3O4; Lateral flow; Limit of Detection; Magnetic; Magnetic Phenomena; Magnetics; Mycotoxins - analysis; Surface-enhanced Raman scattering; Zea mays; Zearalenone; Zearalenone - analysis
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2024.138817

[Display omitted] •Bi-channel SERS-based LFIA strip for simultaneous detection of AFB1 and ZEN.•Magnetic Fe3O4@PEI/AuMBA@Ag-MBA as the active SERS tags.•Magnetic enrichment significantly increased the sensitivity.•High specificity and satisfactory recoveries for AFB1 and ZEN.•Short detection time (20 min) suitable for on-site detection. Aflatoxin B1 (AFB1) and zearalenone (ZEN) are two mycotoxins that often co-occur in corn. A surface-enhanced Raman scattering-based lateral flow immunoassay (SERS-LFIA) that can simultaneously detect AFB1 and ZEN in corn samples was developed employing the core-interlayer-satellite magnetic nanocomposites (Fe3O4@PEI/AuMBA@AgMBA) as dual-functional SERS tags. Under the optimal conditions, the detection ranges of AFB1 and ZEN in corn samples were 0.1–10 μg/kg and 4–400 μg/kg, respectively. Moreover, the test results for two mycotoxins in contaminated corn samples employing the suggested SERS-LFIA was in line with those of the HPLC technique. In view of its satisfactory sensitivity, accuracy, precision and short testing time (20 min), the developed system has a promising application prospect in the on-site simultaneous detection of AFB1 and ZEN.