Can Sb(III)-oxidizing prokaryote also oxidize As(III) under aerobic and anaerobic conditions, and vice versa?

• Published in: Journal of hazardous materials 2024-05, Vol.470, p.134135, Article 134135
• Main Authors: Chen, Xiaoming, Yu, Tingting, Xiao, Linhai, Zeng, Xian-Chun
• Format: Article
• Language: English
• Subjects: Aerobiosis; Anaerobiosis; Antimony - metabolism; As(III) oxidation; As(III)-oxidizing prokaryote (AOP); Bacteria - genetics; Bacteria - metabolism; Oxidation-Reduction; Prokaryotic Cells - metabolism; Sb(III) or As(III) contamination; Sb(III) oxidation; Sb(III)-oxidizing prokaryote (SOP); Soil Microbiology; Soil Pollutants - metabolism
• ISSN: 0304-3894; 1873-3336; 1873-3336
• DOI: 10.1016/j.jhazmat.2024.134135

Sb(III) and As(III) share similar chemical features and coexist in the environment. However, their oxidase enzymes have completely different sequences and structures. This raises an intriguing question: Could Sb(III)-oxidizing prokaryotes (SOPs) also oxidize As(III), and vice versa? Regarding this issue, previous investigations have yielded unclear, incorrect and even conflicting data. This work aims to address this matter. First, we prepared an enriched population of SOPs that comprises 55 different AnoA genes, lacking AioAB and ArxAB genes. We found that these SOPs can oxidize both Sb(III) and As(III) with comparable capabilities. To further confirm this finding, we isolated three cultivable SOP strains that have AnoA gene, but lack AioAB and ArxAB genes. We observed that they also oxidize both Sb(III) and As(III) under both anaerobic and aerobic conditions. Secondly, we obtained an enriched population of As(III)-oxidizing prokaryotes (AOPs) from As-contaminated soils, which comprises 69 different AioA genes, lacking AnoA gene. We observed that the AOP population has significant As(III)-oxidizing activities, but lack detectable Sb(III)-oxidizing activities under both aerobic and anaerobic conditions. Therefore, we convincingly show that SOPs can oxidize As(III), but AOPs cannot oxidize Sb(III). These findings clarify the previous ambiguities, confusion, errors or contradictions regarding how SOPs and AOPs oxidize each other’s substrate. [Display omitted] •Sb(III)-oxidizing and As(III)-oxidizing prokaryotes, SOPs and AOPs, were prepared.•SOPs, with Sb(III) oxidase but no As(III) oxidase, oxidize both Sb(III) and As(III).•Cultivable SOP, with Sb(III) oxidase but no As(III) oxidase, oxidize both Sb(III) and As(III).•AOPs, with As(III) oxidase but no Sb(III) oxidase, oxidize As(III), not Sb(III).•Cultivable AOP, with As(III) oxidase but no Sb(III) oxidase, oxidize As(III), not Sb(III).