Biomimetic Bacterium-like Particles Loaded with Aggregation-Induced Emission Photosensitizers as Plasma Coatings for Implant-Associated Infections

• Published in: ACS applied materials & interfaces 2024-04, Vol.16 (15), p.18449-18458
• Main Authors: Wang, Jianzhong, Ninan, Neethu, Nguyen, Ngoc Huu, Nguyen, Manh Tuong, Sahu, Resmarani, Nguyen, Tien Thanh, Mierczynska-Vasilev, Agnieszka, Vasilev, Krasimir, Truong, Vi Khanh, Tang, Youhong
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - chemistry; Bacteria; Biological and Medical Applications of Materials and Interfaces; Biomimetics; Gram-Negative Bacteria; Gram-Positive Bacteria; Mammals; Photochemotherapy; Photosensitizing Agents - chemistry; Postoperative Complications
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.3c19484

Developing novel antibacterial strategies has become an urgent requisite to overcome the increasing pervasiveness of antimicrobial-resistant bacteria and the advent of biofilms. Aggregation-induced emission-based photosensitizers (AIE PSs) are promising candidates due to their unique photodynamic and photothermal properties. Bioengineering structure-inherent AIE PSs for developing thin film coatings is still an unexplored area in the field of nanoscience. We have adopted a synergistic approach combining plasma technology and AIE PS-based photodynamic therapy to develop coatings that can eradicate bacterial infections. Here, we loaded AIE PSs within biomimetic bacterium-like particles derived from a probiotic strain, Lactobacillus fermentum. These hybrid conjugates are then immobilized on polyoxazoline-coated substrates to develop a bioinspired coating to fight against implant-associated infections. These coatings could selectively kill Gram-positive and Gram-negative bacteria, but not damage mammalian cells. The mechanistic studies revealed that the coatings can generate reactive oxygen species that can rupture the bacterial cell membranes. The mRNA gene expression of proinflammatory cytokines confirmed that they can modulate infection-related immune responses. Thus, this nature-inspired design has opened a new avenue for the fabrication of a next-generation antibacterial coating to reduce infections and associated burdens.