Assessing the impact of Chlorantraniliprole (CAP) pesticide stress on oilseed rape (Brassia campestris L.): Residue dynamics, enzyme activities, and metabolite profiling

• Published in: Pesticide biochemistry and physiology 2024-03, Vol.200, p.105785-105785, Article 105785
• Main Authors: Zhang, Hui, Wang, Pingping, Wang, Jiangfei, Liu, Huijun, Chen, Xiangning
• Format: Article
• Language: English
• Subjects: arginine; biosynthesis; Brassica napus; Brassica napus - metabolism; Chlorantraniliprole; Enzyme activity; enzymes; flavonoids; metabolites; Metabolomics; Oilseed rape; ortho-Aminobenzoates; pesticides; Pesticides - metabolism; proline; Residue; Soil
• ISSN: 0048-3575; 1095-9939
• DOI: 10.1016/j.pestbp.2024.105785

This study investigates the effects of chlorantraniliprole (CAP) pesticide stress on oilseed rape through comprehensive pot experiments. Assessing CAP residue variations in soil and oilseed rape (Brassia campestris L.), enzyme activities (POD, CPR, GST), and differential metabolites, we unveil significant findings. The average CAP residue levels were 18.38–13.70 mg/kg in unplanted soil, 9.94–6.30 mg/kg in planted soil, and 0–4.18 mg/kg in oilseed rape samples, respectively. Soil microbial influences and systemic pesticide translocation into oilseed rape contribute to CAP residue variations. Under the influence of CAP stress, oilseed rape displays escalated enzyme activities (POD, CPR, GST) and manifests 57 differential metabolites. Among these, 32 demonstrate considerable downregulation, mainly impacting amino acids and phenolic compounds, while 25 exhibit noteworthy overexpression, primarily affecting flavonoid compounds. This impact extends to 24 metabolic pathways, notably influencing amide biosynthesis, as well as arginine and proline metabolism. These findings underscore the discernible effects of CAP pesticide stress on oilseed rape. [Display omitted] •This study elucidates the impact of chlorothalonil (CAP) pesticide stress on oilseed rape through rigorous pot experiments.•Notable findings reveal CAP residue variations, enzyme activity (CPR, POD and GST) alterations, and significant differential metabolites in oilseed rape.•The systemic behavior of Brassica napus under CAP stress was revealed, highlighting its enzymatic responses and metabolic pathways affected by pesticide exposure.