Use of 3D printing to integrate microchip electrophoresis with amperometric detection

• Published in: Analytical and bioanalytical chemistry 2024-09, Vol.416 (21), p.4749-4758
• Main Authors: Selemani, Major A., Martin, R. Scott
• Format: Article
• Language: English
• Subjects: 3-D printers; Amperometry; Analytical Chemistry; Biochemistry; Catecholamines; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Continuous flow; Design; Electric potential; Electrical measurement; Electrodes; Electrophoresis; Emerging Trends in Electrochemical Analysis; Fabrication; Food Science; Integrated circuits; Laboratory Medicine; Microelectrodes; Monitoring/Environmental Analysis; Research Paper; Separation; Three dimensional flow; Three dimensional printing; Voltage
• ISSN: 1618-2642; 1618-2650; 1618-2650
• DOI: 10.1007/s00216-024-05260-6

This paper describes the use of PolyJet 3D printing to fabricate microchip electrophoresis devices with integrated microwire electrodes for amperometric detection. The fabrication process involves 3D printing of two separate pieces, a channel layer and an electrode layer. The channel layer is created by 3D printing on a pre-fabricated mold with a T-intersection. For the electrode layer, a stencil design is printed directly on the printing tray and covered with a piece of transparent glass. Microwire electrodes are adhered over the glass piece (guided by underlaying stencil) and a CAD design of the electrode layer is then printed on top of the microwire electrode. After delamination from the glass after printing, the microwire is embedded in the printed piece, with the stencil design ensuring that alignment and positioning of the electrode is reproducible for each print. After a thermal bonding step between the channel layer and electrode layer, a complete electrophoresis device with integrated microelectrodes for amperometric detection results. It is shown that this approach enables different microwire electrodes (gold or platinum) and sizes (100 or 50 µm) to be integrated in an end-channel configuration with no gap between the electrode and the separation channel. These devices were used to separate a mixture of catecholamines and the effect of separation voltage on the potential voltage applied on the working electrode was also investigated. In addition, the effect of electrode size on the number of theoretical plates and limit of detection was studied. Finally, a device that contains different channel heights and a detection electrode was 3D-printed to integrate continuous flow sampling with microchip electrophoresis and amperometric detection.