Semi-quantitative metalloproteinase-8 rapid test for the prediction of adverse pregnancy outcomes in patients with preterm premature rupture of membranes

•Our study introduces the semi-quantitative MMP-8 rapid test, assessing inflammation in amniotic fluid.•Demonstrating a clear correlation with WBC counts, proving its reliability as an indicator of inflammation.•Direct association between the test results and increased risks of intra-amniotic infect...

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Bibliographic Details
Published in:European journal of obstetrics & gynecology and reproductive biology 2024-06, Vol.297, p.65-71
Main Authors: Ji Kim, Hyeon, Choi, Jihyun, Ji Oh, Eun, Lee, Kyong-No, Yoon Park, Jee, Joon Oh, Kyung
Format: Article
Language:English
Subjects:
Intra-amniotic infection
Intra-amniotic inflammation
Metalloproteinase-8
Semi-quantitative, rapid test
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Summary:•Our study introduces the semi-quantitative MMP-8 rapid test, assessing inflammation in amniotic fluid.•Demonstrating a clear correlation with WBC counts, proving its reliability as an indicator of inflammation.•Direct association between the test results and increased risks of intra-amniotic infection and preterm delivery.•Strong positive result significantly shorten amniocentesis-to-delivery interval, raising risk of adverse perinatal outcomes. We aimed to determine whether the semi-quantitative metalloproteinase-8 (MMP-8) bedside test is a worthwhile indicator in reflecting the severity of of intra-amniotic inflammation (IAI) and in predicting adverse pregnancy outcomes. This retrospective cohort study comprised 76 singleton-pregnant women admitted to the Seoul National University Bundang Hospital with a diagnosis of preterm premature rupture of membranes (preterm PROM) between 20 weeks 0 days and 33 weeks 6 days of gestation who underwent trans-abdominal amniocentesis to confirm intra-amniotic infection by positive results for aerobic/anaerobic bacteria, fungi, and genital mycoplasma and evaluate lung maturity. The semi-quantitative MMP-8 rapid test kit employs a colourimetric assay to quantify MMP-8 levels in amniotic fluid (AF), expressing results from 0 to 100 percent. Participants were divided into three groups: group 1, including negative MMP-8 test with colour scale of 0 % (negative, n = 17); group 2, including positive MMP-8 test with colour scale 
ISSN:0301-2115
1872-7654
DOI:10.1016/j.ejogrb.2024.03.044