Protective effect of cellulose and soluble dietary fiber from Saccharina japonica by-products on regulating inflammatory responses, gut microbiota, and SCFAs production in colitis mice

• Published in: International journal of biological macromolecules 2024-05, Vol.267 (Pt 1), p.131214-131214, Article 131214
• Main Authors: Cao, Junhan, Qin, Ling, Zhang, Liping, Wang, Kai, Yao, Mengke, Qu, Changfeng, Miao, Jinlai
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - pharmacology; Cellulose; Cellulose - pharmacology; Colitis - chemically induced; Colitis - metabolism; Dietary Fiber - pharmacology; Disease Models, Animal; Edible Seaweeds; Fatty Acids, Volatile - metabolism; Gastrointestinal Microbiome - drug effects; Gut microbiota; Inflammation - metabolism; Laminaria; Male; Mice; Oxidative Stress - drug effects; Physicochemical properties; Saccharina japonica; SDF; Solubility; Ulcerative colitis
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.131214

This study aimed to investigate the physicochemical properties of soluble dietary fiber (SDF) and cellulose enriched in Saccharina japonica by-products and to evaluate their anti-colitis effects. The water-holding capacity (WHC), swelling capacity (SC), cation exchange capacity (CEC), and antioxidant properties of SDF were superior to cellulose. The ΔH of SDF and cellulose was 340.73 J/g and 134.56 J/g, and the average particle size of them was 43.858 μm and 97.350 μm. The viscosity of SDF was positively correlated with the content. SEM revealed that the microstructure of SDF was porous, whereas cellulose was folded. SDF contained seven monosaccharides such as mannuronic acid and mannose, while cellulose had a single glucose composition. It was also shown that both SDF and cellulose reversed the pathological process of colitis by inhibiting weight loss, preventing colon injury, balancing oxidative stress, and regulating the level of inflammation, with the optimal dose being 1.5 g/kg. The difference was that SDF inhibited the expression of NF-кB and TNF-α, while cellulose up-regulated the expression of PPAR-γ and IL-10. Additionally, SDF could more positively control the expression of ZO-1, whereas cellulose was superior in improving the expression of Occludin. Interestingly, SDF could restore the structure of norank_f_Muribaculaceae and Lachnospiraceae_NK4A136_group to ameliorate ulcerative colitis (UC), whereas cellulose mainly regulated the abundance of norank_f_Muribaculaceae, Faecalibaculum, Bacteroides and unclassified_f__Lachnospiraceae. The production of short-chain fatty acids (SCFAs) was also found to be restored by SDF and cellulose. Overall, SDF and cellulose can be considered important dietary components for treating and preventing UC. [Display omitted]