Isolation and Characterization of Paracoccus maritimus sp. nov., from Intertidal Sediment

• Published in: Current microbiology 2024-05, Vol.81 (5), p.134-134, Article 134
• Main Authors: Yu, Libo, Xu, Guangxin, Wei, Shiping, Lai, Qiliang, Shao, Zongze
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Biotechnology; Cardiolipin; Catalase; Cellulose; Chromatography; Coenzyme Q10; Deoxyribonucleic acid; DNA; Fatty Acids; Gene sequencing; Genomes; Hybridization; Lecithin; Life Sciences; Lipids; Menaquinones; Microbiology; Morphology; New species; Novel Prokaryotic Taxa; Paracoccus; Paracoccus - genetics; Phosphatidylcholine; Phosphatidylethanolamine; Phosphatidylglycerol; Phylogenetics; Phylogeny; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sediment samplers; Sediments; Sodium chloride; Stains & staining
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-024-03637-5

A novel Paracoccus -related strain, designated YLB-12 T , was isolated from a sediment sample from the tidal zone of Shapowei Port, Xiamen, Fujian Province, PR China. The novel strain is a Gram-stain-negative, short, rod-shaped, nonmotile, catalase- and oxidase-positive strain that grows at 10–37 °C and pH 5.0–9.0 in the presence of 0–12.0% (w/v) NaCl. Phylogenetic analysis of the 16S rRNA gene sequences indicated that this strain belongs to the genus Paracoccus and that its highest sequence similarity was to Paracoccus homiensis DD-R11 T (98.5%), followed by Paracoccus zeaxanthinifaciens ATCC 21588 T (97.4%), Paracoccus rhizosphaerae LMG 26205 T (97.2%), Paracoccus beibuensis CGMCC 1.7295 T (97.1%) and Paracoccus halotolerans CFH 90064 T (97.0%). The DNA‒DNA hybridization values between strain YLB-12 T and the five closely related type strains ranged from 20.4 to 22.4%. The genomic G+C content of strain YLB-12 T was 63.7%. In addition to diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and phosphatidylglycerol, the polar lipids of the strain YLB-12 T also consisted of an unidentified glycolipid and four unidentified polar lipids. The cells contained summed feature 8 (C 18: 1 ω 6c /C 18: 1 ω 7c, 62.7%) as the major cellular fatty acid and ubiquinone-10 as the predominant menaquinone. On the basis of its phenotypic and genotypic characteristics, strain YLB-12 T represents a novel species within the genus Paracoccus , for which the name Paracoccus maritimus sp. nov. is proposed. The type strain was YLB-12 T (= MCCC 1A17213 T  = KCTC 82197 T ).