Sodium alginate/carboxycellulose/polydopamine composite microspheres for rapid hemostasis of deep irregular wounds

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2024-06, Vol.238, p.113905-113905, Article 113905
• Main Authors: Hu, Junjie, Hu, Yinchun, Kang, Min, Liu, Xuanyu, Wu, Baogang, Wang, Lining, Wei, Yan, Huang, Di
• Format: Article
• Language: English
• Subjects: Carboxycellulose; Deep irregular wounds; Hemostatic microspheres; Polydopamine; Sodium alginate
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2024.113905

Hemostasis of deep irregular wounds is a severe problem in clinical practice. The development of rapid-acting hemostatic agents for deep and irregular wound is urgently needed. Here, sodium alginate/carboxycellulose/polydopamine (SA/CNF/PDA) microspheres was prepared by reverse emulsification and crosslinking with Ca2+, and SA/CNF/PDA composite hemostatic microspheres with porous structure were obtained by freeze-drying. SA/CNF/PDA composite hemostatic microspheres exhibited excellent porosity and water absorption which could rapidly absorb blood on the wound surface. Moreover, SA/CNF/PDA composite microspheres demonstrated remarkable hemostatic capabilities both in vitro and in vivo. It exhibited strong hemostatic performance in models of mouse tail-break and liver damage. Especially in liver injury model, it was completely hemostatic in 95 s, and blood loss (19.3 mg). The hemostatic efficacy of the SA/CNF/PDA composite microspheres was amplified through the stimulation of both exogenous and endogenous coagulation pathways. Therefore, SA/CNF/PDA composite hemostatic microspheres are suitable for rapid hemostasis of deep irregular wounds which are potential rapid hemostatic material for surgical application. •SA/CNF/PDA hemostatic microsphere was obtained by reverse emulsification and freeze-drying.•SA/CNF/PDA microsphere stopped bleeding within 95 s in liver injury model.•The activation of exogenous and endogenous coagulation pathways were activated.