Fluorogenic cell surface glycan labelling with fluorescence molecular rotor dyes and nucleic acid stains

We show that covalent labelling of sialic acids on live cell surfaces or mucin increases the fluorescence of the fluorescence molecular rotors (FMRs) CCVJ, Cy3 and thioazole orange, enabling wash-free imaging of cell surfaces. Dual labelling with an FMR and an environmentally insensitive dye allows...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2024-04, Vol.6 (36), p.4785-4788
Main Authors: Koçak, Alen, Homer, Amal K, Feida, Antonia, Telschow, Florian, Gorenflos López, Jacob L, Baydaro lu, Cihan, Gradzielski, Michael, Hackenberger, Christian P. R, Alexiev, Ulrike, Seitz, Oliver
Format: Article
Language:English
Subjects:
Crosslinking
Dyes
Fluorescence
Glycan
Labeling
Nucleic acids
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Summary:We show that covalent labelling of sialic acids on live cell surfaces or mucin increases the fluorescence of the fluorescence molecular rotors (FMRs) CCVJ, Cy3 and thioazole orange, enabling wash-free imaging of cell surfaces. Dual labelling with an FMR and an environmentally insensitive dye allows detection of changes that occur, for example, when cross-linking is altered. Covalent labelling of sialic acids on live cell surfaces or mucin with fluorescence molecular rotors enables wash-free imaging and provides information about changes occurring to cell surfaces.
ISSN:1359-7345
1364-548X
DOI:10.1039/d4cc00884g