Molecular heterogeneity of quiescent melanocyte stem cells revealed by single‐cell RNA‐sequencing

Melanocyte stem cells (McSCs) of the hair follicle are a rare cell population within the skin and are notably underrepresented in whole‐skin, single‐cell RNA sequencing (scRNA‐seq) datasets. Using a cell enrichment strategy to isolate KIT+/CD45− cells from the telogen skin of adult female C57BL/6J m...

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Published in:Pigment cell and melanoma research 2024-07, Vol.37 (4), p.480-495
Main Authors: Palmer, Joseph W., Kumar, Nilesh, An, Luye, White, Andrew C., Mukhtar, M. Shahid, Harris, Melissa L.
Format: Article
Language:English
Subjects:
Animals
CD34 antigen
CD45 antigen
Cell Differentiation
Female
Gene sequencing
Genetic Heterogeneity
Hair Follicle - cytology
Hair Follicle - metabolism
Heterogeneity
Immune privilege
melanocyte stem cell
Melanocytes - cytology
Melanocytes - metabolism
Mice
Mice, Inbred C57BL
mouse skin
Neural crest
quiescence
scRNA‐seq
Sequence Analysis, RNA
Single-Cell Analysis
Skin
Stem cells
Stem Cells - cytology
Stem Cells - metabolism
Subpopulations
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Summary:Melanocyte stem cells (McSCs) of the hair follicle are a rare cell population within the skin and are notably underrepresented in whole‐skin, single‐cell RNA sequencing (scRNA‐seq) datasets. Using a cell enrichment strategy to isolate KIT+/CD45− cells from the telogen skin of adult female C57BL/6J mice, we evaluated the transcriptional landscape of quiescent McSCs (qMcSCs) at high resolution. Through this evaluation, we confirmed existing molecular signatures for qMcCS subpopulations (e.g., Kit+, Cd34+/−, Plp1+, Cd274+/−, Thy1+, Cdh3+/−) and identified novel qMcSC subpopulations, including two that differentially regulate their immune privilege status. Within qMcSC subpopulations, we also predicted melanocyte differentiation potential, neural crest potential, and quiescence depth. Taken together, the results demonstrate that the qMcSC population is heterogeneous and future studies focused on investigating changes in qMcSCs should consider changes in subpopulation composition. Quiescent melanocyte stem cells are functionally, molecularly and anatomically heterogeneous. High resolution single cell RNA sequencing resolves established and novel observations into a single working model.
ISSN:1755-1471
1755-148X
1755-148X
DOI:10.1111/pcmr.13169