Bone Morphogenetic Protein 2 Enhances Porcine Beige Adipogenesis via AKT/mTOR and MAPK Signaling Pathways

• Published in: International journal of molecular sciences 2024-04, Vol.25 (7), p.3915
• Main Authors: Liu, Jiali, Jiang, Yao, Chen, Chuanhe, Zhang, Lilan, Wang, Jiahao, Yang, Chunhuai, Wu, Tianwen, Yang, Shulin, Tao, Cong, Wang, Yanfang
• Format: Article
• Language: English
• Subjects: Adipocytes; Adipogenesis - genetics; Animals; Body fat; Body temperature; Bone Morphogenetic Protein 2 - genetics; Bone morphogenetic proteins; Cell differentiation; Cell growth; Cold; Efficiency; Hogs; Lipids; Metabolism; Obesity; PPAR gamma; Proteins; Proto-Oncogene Proteins c-akt; RNA; Signal Transduction; Swine; TOR Serine-Threonine Kinases - genetics; Transcription factors
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25073915

Bone morphogenetic protein 2 (BMP2) has been reported to regulate adipogenesis, but its role in porcine beige adipocyte formation remains unclear. Our data reveal that is significantly induced at the early stages of porcine beige adipocyte differentiation. Additionally, supplementing rhBMP2 during the early stages, but not the late stages of differentiation, significantly enhances porcine SVF adipogenesis, thermogenesis, and proliferation. Furthermore, compared to the empty plasmid-transfected-SVFs, -overexpressed SVFs had the enhanced lipid accumulation and thermogenesis, while knockdown of in SVFs exhibited the opposite effect. The RNA-seq of the above three types of cells revealed the enrichment of the annotation of thermogenesis, brown cell differentiation, etc. In addition, the analysis also highlights the significant enrichment of cell adhesion, the MAPK cascade, and PPARγ signaling. Mechanistically, positively regulates the adipogenic and thermogenic capacities of porcine beige adipocytes by activating PPARγ expression through AKT/mTOR and MAPK signaling pathways.