Biomarker and proteome analysis of milk from dairy cows with clinical mastitis: Determining the effect of different bacterial pathogens on the response to infection

• Published in: Research in veterinary science 2024-06, Vol.172, p.105240-105240, Article 105240
• Main Authors: O'Reilly, Emily L., Viora, Lorenzo, Malcata, Francisco, Pepler, P. Theo, Zadoks, Ruth, Brady, Nicola, Hanh, Han Quang, McLaughlin, Mark, Horvatic, Anita, Gelemanovic, Andrea, Kules, Josipa, Mrljak, Vladimir, Eckersall, P. David
• Format: Article
• Language: English
• Subjects: Acute phase; Classification tree diagnostic; Dairy biomarker; Mastitis
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2024.105240

Antimicrobial usage (AMU) could be reduced by differentiating the causative bacteria in cases of clinical mastitis (CM) as either Gram-positive or Gram-negative bacteria or identifying whether the case is culture-negative (no growth, NG) mastitis. Immunoassays for biomarker analysis and a Tandem Mass Tag (TMT) proteomic investigation were employed to identify differences between samples of milk from cows with CM caused by different bacteria. A total of 94 milk samples were collected from cows diagnosed with CM across seven farms in Scotland, categorized by severity as mild (score 1), moderate (score 2), or severe (score 3). Bovine haptoglobin (Hp), milk amyloid A (MAA), C-reactive protein (CRP), lactoferrin (LF), α-lactalbumin (LA) and cathelicidin (CATHL) were significantly higher in milk from cows with CM, regardless of culture results, than in milk from healthy cows (all P-values