Unveiling the Biocontrol Potential of Rhizoplane Bacillus Species against Sugarcane Fusarium Wilt through Biochemical and Molecular Analysis

• Published in: Brazilian journal of microbiology 2024-06, Vol.55 (2), p.1883-1896
• Main Authors: Chetan, K. K., Kishore Varma, P., Chandrasekhar, V., Anil Kumar, P., Vasanthi, V., Vamshi Krishna, G.
• Format: Article
• Language: English
• Subjects: Antibiosis; Bacillus - classification; Bacillus - genetics; Bacillus - isolation & purification; Bacillus - metabolism; Bacillus - physiology; Biological Control Agents; Biomedical and Life Sciences; Food Microbiology; Fusarium - genetics; Fusarium - physiology; Life Sciences; Medical Microbiology; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Mycology; Phylogeny; Plant Diseases - microbiology; Plant Diseases - prevention & control; Rhizosphere; Saccharum - microbiology; Soil and Agricultural Microbiology - Research Paper; Soil Microbiology
• ISSN: 1517-8382; 1678-4405; 1678-4405
• DOI: 10.1007/s42770-024-01307-z

Background Biocontrol is regarded as a viable alternate technique for managing sugarcane wilt disease caused by Fusarium sacchari. Many fungal antagonists against F. sacchari , have been reported, but the potential of bacterial antagonists was explored to a limited extent, so the present study evaluated the antagonistic potential of rhizoplane Bacillus species and their mode of action. Results A total of twenty Bacillus isolates from the rhizoplane of commercially grown sugarcane varieties were isolated. The potential isolate SRB2 had shown inhibition of 52.30, 33.33, & 44.44% and SRB20 of 35.00, 33.15, & 36.85% in direct, indirect, and remote confrontation respectively against F. sacchari . The effective strains were identified as Bacillus inaquosorum strain SRB2 and B. vallismortis strain SRB20, by PCR amplification of 16S-23S intergenic region. The biochemical studies on various direct and indirect biocontrol mechanisms revealed the production of IAA, Protease, Cellulase, Siderophores, and P solubilization. The molecular analysis revealed the presence of antimicrobial peptides biosynthetic genes like fenD (Fengycin), bmyB (Bacyllomicin) ituC (Iturin) and spaS (Subtilin) which provided a competitive edge to these isolates compared to other Bacillus strains. Under greenhouse experiments, the sett bacterization with SRB2, significantly ( P   70% followed by SRB20 in F. sacchari inoculated pots. Conclusion The study revealed that the isolates B. inaquosorum SRB2 and B. vallismortis SRB20 can be used as potential bioagents against sugarcane Fusarium wilt.